Detailed information of TA system TA066518

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 94298..94821
• Replicon: plasmid unnamed5
• Accession: NZ_CP017429
• Organism: Staphylococcus simulans strain MR3

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: -
• Locus tag: BI282_RS13485
• Protein ID: WP_106421233.1
• Coordinates: 94555..94821 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: -
• Locus tag: BI282_RS13480
• Protein ID: WP_106421232.1
• Coordinates: 94298..94555 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BI282_RS13450	89885..90490	+	606	WP_106421227.1	sporulation delaying protein family toxin	-
BI282_RS13455	90493..90879	+	387	WP_106421228.1	DUF393 domain-containing protein	-
BI282_RS13460	90920..91561	-	642	WP_106421229.1	SdpI family protein	-
BI282_RS13465	91580..91831	-	252	WP_106421230.1	autorepressor SdpR family transcription factor	-
BI282_RS13470	92899..93381	+	483	Protein_124	recombinase family protein	-
BI282_RS13480	94298..94555	+	258	WP_106421232.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
BI282_RS13485	94555..94821	+	267	WP_106421233.1	Txe/YoeB family addiction module toxin	Toxin
BI282_RS13490	95428..96102	+	675	WP_103370613.1	IS6 family transposase	-
BI282_RS13495	96112..96957	-	846	WP_106421234.1	protein rep	-
BI282_RS13500	97496..98041	-	546	WP_105988897.1	hypothetical protein	-
BI282_RS14030	99138..99281	-	144	WP_106421236.1	Rep protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	lnu(A)	-	1..146786	146786

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (25-87)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-68)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10642.14 Da
• Isoelectric Point: 10.0751

>T66518 WP_106421233.1 NZ_CP017429:94555-94821 [Staphylococcus simulans]
MSNYSISIKNSAKSDLKKIQQSQLKSHFLKIVEILKIDPYYPNQSFEKLQPKHLGRYSRRINHQHRVVYTVDEERKEVYI
FSAWSHYE

Nucleotide

• Length: 267 bp

>T66518 NZ_CP017429:94555-94821 [Staphylococcus simulans]
ATGAGTAACTACAGTATTTCAATTAAAAATTCGGCGAAAAGTGATTTAAAAAAAATACAGCAGTCACAACTTAAAAGTCA
TTTTTTAAAAATTGTAGAAATATTAAAGATAGACCCATATTATCCAAACCAATCATTTGAAAAGTTACAACCAAAACATT
TAGGGCGCTATTCAAGGCGTATCAATCATCAGCATAGAGTAGTTTATACAGTCGATGAAGAAAGAAAAGAAGTATATATT
TTTTCTGCGTGGTCTCATTATGAATAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9832.82 Da
• Isoelectric Point: 4.3646

>AT66518 WP_106421232.1 NZ_CP017429:94298-94555 [Staphylococcus simulans]
MNIKTPTNARKEFYELLKQVNNNHEPVIISGSKSENNAVIVSQKDWNSIQETLFLESTGTMDKVREREKDDSGYTNIDDL
DWDNL

Nucleotide

• Length: 258 bp

>AT66518 NZ_CP017429:94298-94555 [Staphylococcus simulans]
ATGAATATTAAAACTCCTACAAATGCGAGAAAAGAGTTTTACGAGCTGTTAAAACAAGTTAATAATAATCATGAGCCTGT
AATTATAAGTGGCTCTAAAAGTGAAAATAACGCAGTTATTGTAAGTCAAAAAGATTGGAATAGTATTCAAGAAACGTTGT
TTCTTGAATCAACTGGCACTATGGATAAAGTAAGAGAACGTGAAAAAGATGATAGTGGTTATACAAATATTGATGATTTA
GATTGGGATAACTTGTAA