Detailed information of TA system TA066512

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 4994358..4994981
• Replicon: chromosome
• Accession: NZ_CP017420
• Organism: Delftia tsuruhatensis strain CM13

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: BI380_RS23070
• Protein ID: WP_043792262.1
• Coordinates: 4994358..4994639 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A1D8J5P8
• Locus tag: BI380_RS23075
• Protein ID: WP_046238967.1
• Coordinates: 4994682..4994981 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BI380_RS23045	4989714..4990577	+	864	WP_016448674.1	branched-chain amino acid ABC transporter permease	-
BI380_RS23050	4990578..4991552	+	975	WP_046238969.1	branched-chain amino acid ABC transporter permease	-
BI380_RS23055	4991539..4992303	+	765	WP_016452146.1	ABC transporter ATP-binding protein	-
BI380_RS23060	4992290..4992997	+	708	WP_046238968.1	ABC transporter ATP-binding protein	-
BI380_RS23065	4993053..4994228	+	1176	WP_013800139.1	ABC transporter substrate-binding protein	-
BI380_RS23070	4994358..4994639	+	282	WP_043792262.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BI380_RS23075	4994682..4994981	+	300	WP_046238967.1	HigA family addiction module antidote protein	Antitoxin
BI380_RS23080	4995074..4995847	-	774	WP_016448669.1	SDR family oxidoreductase	-
BI380_RS23085	4996003..4996809	-	807	WP_016448668.1	IclR family transcriptional regulator	-
BI380_RS23090	4996854..4997627	-	774	WP_016448667.1	ferredoxin--NADP reductase	-
BI380_RS23095	4997889..4998824	-	936	WP_070080799.1	LysR family transcriptional regulator	-
BI380_RS23100	4998917..4999708	+	792	WP_070080800.1	SDR family oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11038.66 Da
• Isoelectric Point: 9.3583

>T66512 WP_043792262.1 NZ_CP017420:4994358-4994639 [Delftia tsuruhatensis]
MTIQSFKCPDTEQLFQQRQVRRWSSIEVVALRKLRMLHAAHVLQDLRVPPGNRLEALHGDRKGQHSIRINDQWRVCFVWT
HEGPSQLEIVDYH

Nucleotide

• Length: 282 bp

>T66512 NZ_CP017420:4994358-4994639 [Delftia tsuruhatensis]
ATGACGATCCAGTCATTCAAGTGCCCCGACACCGAGCAACTGTTCCAGCAGAGGCAGGTGCGTCGGTGGAGCAGCATTGA
AGTGGTTGCGCTGCGCAAGCTTCGGATGCTGCATGCCGCTCATGTGCTTCAGGACTTGAGGGTCCCGCCCGGAAACCGGC
TGGAGGCGCTGCACGGAGACCGCAAGGGGCAGCACAGCATCCGCATCAATGACCAGTGGCGCGTGTGCTTTGTGTGGACC
CATGAAGGCCCGAGTCAGCTGGAGATCGTCGATTACCACTGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10962.51 Da
• Isoelectric Point: 4.5183

>AT66512 WP_046238967.1 NZ_CP017420:4994682-4994981 [Delftia tsuruhatensis]
MNKPLNLPTPGEILATEFLEPMGLTQYRLAQAIHVPQTRIAAIAKNGRSITADTALRLARFFGTSVEFWMNLQSQYDADI
AREAIGDDLESIEPFALQA

Nucleotide

• Length: 300 bp

>AT66512 NZ_CP017420:4994682-4994981 [Delftia tsuruhatensis]
ATGAACAAACCTTTGAACCTCCCCACGCCAGGCGAAATCCTGGCCACTGAATTCCTGGAGCCCATGGGCCTGACGCAATA
CCGCCTGGCCCAGGCCATCCATGTCCCGCAGACACGCATCGCGGCCATTGCGAAGAACGGCCGCTCCATCACGGCGGATA
CGGCGCTGCGGCTGGCGCGCTTCTTTGGCACGTCCGTGGAGTTCTGGATGAATCTGCAAAGCCAGTACGACGCCGATATC
GCGCGCGAGGCCATAGGCGACGACCTGGAGTCCATCGAGCCCTTTGCTCTGCAGGCTTGA

Structures

Antitoxin

• AlphaFold DB: A0A1D8J5P8