Detailed information of TA system TA066509

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3984850..3985524
• Replicon: chromosome
• Accession: NZ_CP017420
• Organism: Delftia tsuruhatensis strain CM13

Toxin (Protein)

• Gene name: graT
• Uniprot ID: -
• Locus tag: BI380_RS18540
• Protein ID: WP_043789322.1
• Coordinates: 3984850..3985131 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: BI380_RS18545
• Protein ID: WP_012203141.1
• Coordinates: 3985213..3985524 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BI380_RS18520	3980516..3981082	-	567	WP_043789355.1	type I-F CRISPR-associated endoribonuclease Cas6/Csy4	-
BI380_RS18525	3981088..3982116	-	1029	WP_016451881.1	type I-F CRISPR-associated protein Csy3	-
BI380_RS18530	3982171..3983289	-	1119	WP_046240414.1	type I-F CRISPR-associated protein Csy2	-
BI380_RS18535	3983286..3984683	-	1398	WP_046240415.1	type I-F CRISPR-associated protein Csy1	-
BI380_RS18540	3984850..3985131	+	282	WP_043789322.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BI380_RS18545	3985213..3985524	+	312	WP_012203141.1	HigA family addiction module antidote protein	Antitoxin
BI380_RS18550	3985528..3989016	-	3489	WP_046240416.1	type I-F CRISPR-associated helicase Cas3	-
BI380_RS18555	3989013..3990035	-	1023	WP_046240417.1	type I-F CRISPR-associated endonuclease Cas1	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-93)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-98)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10721.30 Da
• Isoelectric Point: 8.5442

>T66509 WP_043789322.1 NZ_CP017420:3984850-3985131 [Delftia tsuruhatensis]
MAITSFKCPETQRLFTSGKTRRFANIQSVAERKLAQLDAAVTLEFLRSPPGNRLEMLVGDRQGQHSIRINDQWRLCFVWT
EKGVVDVEIVDYH

Nucleotide

• Length: 282 bp

>T66509 NZ_CP017420:3984850-3985131 [Delftia tsuruhatensis]
ATGGCGATCACTTCCTTCAAGTGCCCGGAAACACAGCGCCTGTTCACCAGCGGCAAGACCCGCCGCTTCGCCAACATCCA
GAGCGTGGCGGAGCGCAAGCTGGCTCAGCTGGATGCGGCTGTGACACTTGAATTTCTGCGCAGCCCTCCTGGCAATCGCC
TGGAAATGCTCGTGGGAGATCGGCAGGGCCAGCACAGCATCCGCATCAACGACCAGTGGCGCCTCTGCTTTGTCTGGACG
GAAAAGGGAGTGGTTGACGTAGAGATAGTGGATTACCACTGA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11615.32 Da
• Isoelectric Point: 8.6927

>AT66509 WP_012203141.1 NZ_CP017420:3985213-3985524 [Delftia tsuruhatensis]
MLKNGMRPIHPGEVLREDYLKPMDMSANALARHLHVPASRVNDIVLERRGITADTALRLSRFFGGDAQSWLNLQTAYDLR
TAELNAELQASVRQVHPMQLHTA

Nucleotide

• Length: 312 bp

>AT66509 NZ_CP017420:3985213-3985524 [Delftia tsuruhatensis]
ATGCTTAAAAACGGCATGCGCCCCATACACCCAGGTGAAGTCCTGCGCGAGGACTACCTGAAGCCCATGGACATGAGCGC
CAACGCGCTGGCCAGGCACCTGCACGTTCCCGCCTCGCGCGTGAACGACATCGTGCTGGAGCGGCGCGGCATCACGGCCG
ACACGGCGCTGCGGCTGAGCCGCTTTTTTGGGGGCGACGCCCAGTCCTGGCTGAACCTTCAGACCGCCTACGACCTGCGC
ACGGCAGAGCTGAACGCGGAGTTGCAGGCGTCCGTGAGGCAAGTTCATCCGATGCAGCTGCATACCGCCTGA