Detailed information of TA system TA066456

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5295653..5296248
• Replicon: chromosome
• Accession: NZ_CP017306
• Organism: Pseudomonas aeruginosa strain PA_150577

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A241XLJ5
• Locus tag: AXX03_RS25205
• Protein ID: WP_003117425.1
• Coordinates: 5295970..5296248 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AXX03_RS25200
• Protein ID: WP_003113527.1
• Coordinates: 5295653..5295958 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AXX03_RS25150	5290746..5290997	-	252	WP_003124954.1	hypothetical protein	-
AXX03_RS25160	5291118..5291552	-	435	WP_096067054.1	DNA-binding protein	-
AXX03_RS25170	5292091..5292381	-	291	WP_034050448.1	DUF5447 family protein	-
AXX03_RS25175	5292557..5292829	-	273	WP_004352675.1	hypothetical protein	-
AXX03_RS30155	5293474..5293644	-	171	WP_153576376.1	hypothetical protein	-
AXX03_RS25190	5293664..5294275	+	612	WP_079392501.1	hypothetical protein	-
AXX03_RS25195	5294277..5295278	+	1002	WP_034050450.1	DUF262 domain-containing protein	-
AXX03_RS25200	5295653..5295958	-	306	WP_003113527.1	HigA family addiction module antidote protein	Antitoxin
AXX03_RS25205	5295970..5296248	-	279	WP_003117425.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AXX03_RS25215	5296577..5298805	+	2229	WP_003113525.1	TonB-dependent receptor	-
AXX03_RS25220	5298875..5299522	-	648	WP_003095021.1	carbonate dehydratase	-
AXX03_RS25225	5299584..5300822	-	1239	WP_014603324.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10701.28 Da
• Isoelectric Point: 8.5576

>T66456 WP_003117425.1 NZ_CP017306:c5296248-5295970 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAARELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T66456 NZ_CP017306:c5296248-5295970 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTAGGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11247.81 Da
• Isoelectric Point: 5.0747

>AT66456 WP_003113527.1 NZ_CP017306:c5295958-5295653 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT66456 NZ_CP017306:c5295958-5295653 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTTGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: A0A241XLJ5