Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 4666303..4666984 Replicon chromosome
Accession NZ_CP017306
Organism Pseudomonas aeruginosa strain PA_150577

Toxin (Protein)


Gene name higB Uniprot ID V6AKP0
Locus tag AXX03_RS22240 Protein ID WP_003111825.1
Coordinates 4666619..4666984 (-) Length 122 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A1C7BMJ2
Locus tag AXX03_RS22235 Protein ID WP_003159602.1
Coordinates 4666303..4666626 (-) Length 108 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AXX03_RS22195 4662262..4662945 - 684 WP_003120766.1 TetR/AcrR family transcriptional regulator -
AXX03_RS22200 4663033..4663911 + 879 WP_014603214.1 hypothetical protein -
AXX03_RS22210 4664514..4664741 + 228 WP_014603215.1 hypothetical protein -
AXX03_RS22215 4664756..4665298 - 543 WP_019396018.1 tyrosine-type recombinase/integrase -
AXX03_RS22220 4665406..4665681 - 276 WP_004353146.1 hypothetical protein -
AXX03_RS22225 4665681..4666013 - 333 WP_021205399.1 hypothetical protein -
AXX03_RS22230 4666068..4666187 + 120 Protein_4291 IS5/IS1182 family transposase -
AXX03_RS22235 4666303..4666626 - 324 WP_003159602.1 XRE family transcriptional regulator Antitoxin
AXX03_RS22240 4666619..4666984 - 366 WP_003111825.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
AXX03_RS30265 4667275..4667643 - 369 WP_162471085.1 hypothetical protein -
AXX03_RS22250 4667656..4667928 + 273 WP_014603216.1 hypothetical protein -
AXX03_RS22255 4667958..4668383 - 426 WP_003116492.1 VOC family protein -
AXX03_RS22260 4668484..4669368 + 885 WP_003085663.1 LysR family transcriptional regulator -
AXX03_RS22265 4669341..4670294 - 954 WP_003085661.1 LysR family transcriptional regulator -
AXX03_RS22270 4670515..4670949 + 435 WP_003116494.1 RidA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-106)

Antitoxin

Cro/C1-type helix-turn-helix domain

(33-63)


Sequences

Toxin        


Download         Length: 122 a.a.        Molecular weight: 13880.24 Da        Isoelectric Point: 4.8219

>T66455 WP_003111825.1 NZ_CP017306:c4666984-4666619 [Pseudomonas aeruginosa]
VAWDIEYTDEFGDWWGSLSEDEQESLAVTVRLLEERGPSLGHPHSSGINGSRHGHMRELRTQHGGRPFRTLYAFDPRRSA
ILLIGGDKTGDDRWYELNVPIADRLYDEHLHQLREEGLIDG

Download         Length: 366 bp

>T66455 NZ_CP017306:c4666984-4666619 [Pseudomonas aeruginosa]
GTGGCGTGGGATATCGAGTACACCGACGAGTTCGGTGATTGGTGGGGTTCTCTGTCCGAGGATGAGCAGGAGTCGCTAGC
TGTGACGGTTCGCTTGCTTGAGGAGCGGGGGCCATCCTTGGGGCACCCCCACAGCAGCGGCATCAATGGCTCCCGCCATG
GCCATATGAGAGAGCTACGGACTCAACACGGCGGGCGACCTTTCCGAACGCTTTACGCGTTTGATCCCAGACGCTCCGCG
ATACTGCTGATCGGTGGCGACAAGACTGGCGATGATCGTTGGTACGAATTGAACGTGCCCATTGCCGACCGCCTCTATGA
CGAACACTTGCACCAGCTCCGTGAGGAGGGACTGATCGATGGCTAA

Antitoxin


Download         Length: 108 a.a.        Molecular weight: 11882.71 Da        Isoelectric Point: 6.4751

>AT66455 WP_003159602.1 NZ_CP017306:c4666626-4666303 [Pseudomonas aeruginosa]
MAKKFSDLRAQMSPEGQARVEAKAQELLAEMPLNELRQARGLSQKMLSEVLHVQQPAIAKMERRTDMYISTLRSHIEAMG
GQLEVIARFPDGAVKISNFADIEEAGA

Download         Length: 324 bp

>AT66455 NZ_CP017306:c4666626-4666303 [Pseudomonas aeruginosa]
ATGGCTAAGAAATTCTCCGACCTTCGGGCGCAAATGTCGCCTGAGGGACAGGCCCGCGTCGAGGCCAAGGCCCAGGAACT
GTTGGCTGAAATGCCCCTGAATGAGTTGCGCCAGGCTAGAGGCTTGTCGCAGAAGATGCTGTCTGAAGTGCTGCACGTGC
AGCAGCCGGCAATTGCCAAAATGGAGCGGCGTACGGATATGTACATCTCCACCTTGCGCAGCCACATCGAAGCCATGGGC
GGCCAACTGGAAGTGATTGCACGCTTCCCGGATGGCGCGGTGAAAATCAGCAACTTCGCGGATATCGAAGAGGCGGGCGC
CTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V6AKP0

Antitoxin

Source ID Structure
AlphaFold DB A0A1C7BMJ2

References