Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 1715515..1716161 Replicon chromosome
Accession NZ_CP017305
Organism Chlorobaculum limnaeum strain DSM 1677

Toxin (Protein)


Gene name higB Uniprot ID A0A1D8D0T0
Locus tag BIU88_RS07635 Protein ID WP_069810128.1
Coordinates 1715841..1716161 (-) Length 107 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A1D8CYM9
Locus tag BIU88_RS07630 Protein ID WP_069810126.1
Coordinates 1715515..1715841 (-) Length 109 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BIU88_RS07610 1710969..1712105 - 1137 WP_069810121.1 PLP-dependent aspartate aminotransferase family protein -
BIU88_RS07615 1712313..1712738 + 426 WP_157098516.1 DUF4395 family protein -
BIU88_RS07620 1713216..1714442 + 1227 WP_069808623.1 ISL3 family transposase -
BIU88_RS13410 1714406..1714807 - 402 WP_157098392.1 hypothetical protein -
BIU88_RS07630 1715515..1715841 - 327 WP_069810126.1 helix-turn-helix transcriptional regulator Antitoxin
BIU88_RS07635 1715841..1716161 - 321 WP_069810128.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
BIU88_RS07640 1716449..1717591 + 1143 WP_069810130.1 aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme -
BIU88_RS07645 1717603..1718535 + 933 WP_069810132.1 cysteine synthase A -
BIU88_RS07650 1718894..1719103 + 210 WP_069810134.1 sulfur carrier protein ThiS -
BIU88_RS07655 1719138..1719917 + 780 WP_069810136.1 thiazole synthase -
BIU88_RS07660 1719914..1720981 + 1068 WP_069810138.1 2-iminoacetate synthase ThiH -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(15-102)

Antitoxin

Cro/C1-type helix-turn-helix domain

(44-95)


Sequences

Toxin        


Download         Length: 107 a.a.        Molecular weight: 12559.79 Da        Isoelectric Point: 10.3680

>T66448 WP_069810128.1 NZ_CP017305:c1716161-1715841 [Chlorobaculum limnaeum]
MSYEIEYFHPRIEAELDRWPSSIRADFRKISLLLVECGPQVRMPYSKAMGDGLFEMRPKGRDGIGRVFYCYVKGKRIIIL
HSFIKKSQKTPHKELQLARKRMQEVQ

Download         Length: 321 bp

>T66448 NZ_CP017305:c1716161-1715841 [Chlorobaculum limnaeum]
ATGAGCTATGAAATCGAATATTTTCATCCTCGCATCGAGGCGGAGCTCGACCGGTGGCCGTCGAGTATCAGGGCGGATTT
CAGAAAAATTTCGCTGCTGTTGGTGGAGTGCGGCCCGCAAGTCCGGATGCCGTATTCCAAAGCTATGGGCGACGGATTGT
TCGAGATGAGGCCGAAAGGCAGGGACGGTATCGGCAGAGTGTTCTACTGCTATGTGAAAGGCAAAAGGATCATCATTTTG
CATAGTTTCATCAAAAAGTCTCAGAAGACGCCACACAAAGAGTTGCAGCTTGCCCGAAAAAGAATGCAGGAGGTTCAATA
A

Antitoxin


Download         Length: 109 a.a.        Molecular weight: 12065.84 Da        Isoelectric Point: 7.9753

>AT66448 WP_069810126.1 NZ_CP017305:c1715841-1715515 [Chlorobaculum limnaeum]
MEDLKYQPVRYDVAAAIKKDLENEEFRKEYEALEPKYALIRELLEARKNSGMTQEAVASKIGTTKSAISRLEGGSQHAPS
IATLRKYAEAVGCELVIRLEPKKKPADA

Download         Length: 327 bp

>AT66448 NZ_CP017305:c1715841-1715515 [Chlorobaculum limnaeum]
ATGGAAGATTTGAAATATCAGCCGGTGCGCTATGATGTTGCGGCAGCTATCAAGAAGGATCTTGAAAACGAGGAGTTTCG
CAAGGAGTATGAAGCGCTGGAGCCGAAGTACGCGCTGATCCGGGAGCTGCTTGAAGCGCGCAAGAACTCCGGCATGACGC
AGGAGGCGGTAGCCAGCAAGATCGGCACGACCAAAAGCGCCATCTCCCGGCTGGAGGGCGGCAGCCAACATGCGCCATCC
ATCGCCACGCTGAGGAAATATGCCGAGGCGGTTGGCTGTGAATTGGTGATCAGGCTGGAGCCGAAAAAGAAACCCGCCGA
CGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1D8D0T0

Antitoxin

Source ID Structure
AlphaFold DB A0A1D8CYM9

References