Detailed information of TA system TA066443

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 546022..546528
• Replicon: chromosome
• Accession: NZ_CP017297
• Organism: Brachybacterium sp. P6-10-X1

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: -
• Locus tag: BH708_RS02390
• Protein ID: WP_076806323.1
• Coordinates: 546022..546276 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: -
• Locus tag: BH708_RS02395
• Protein ID: WP_076806325.1
• Coordinates: 546277..546528 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BH708_RS02375	542277..543434	+	1158	WP_076806317.1	hypothetical protein	-
BH708_RS02380	543431..543808	+	378	WP_076806319.1	hypothetical protein	-
BH708_RS02385	543814..545982	+	2169	WP_076806321.1	DEAD/DEAH box helicase	-
BH708_RS02390	546022..546276	-	255	WP_076806323.1	Txe/YoeB family addiction module toxin	Toxin
BH708_RS02395	546277..546528	-	252	WP_076806325.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
BH708_RS02400	546674..547744	+	1071	WP_157235669.1	hypothetical protein	-
BH708_RS19675	547810..548442	+	633	WP_076806328.1	hypothetical protein	-
BH708_RS02410	548688..549500	-	813	WP_076806330.1	SRPBCC domain-containing protein	-
BH708_RS02415	550039..550695	-	657	WP_083713215.1	hypothetical protein	-
BH708_RS19530	550695..551159	-	465	WP_083713217.1	glycosyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (3-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (2-69)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 9961.39 Da
• Isoelectric Point: 9.4413

>T66443 WP_076806323.1 NZ_CP017297:c546276-546022 [Brachybacterium sp. P6-10-X1]
VRLVWDRSAWEDYKHWQTADRKILKRINTLIDACLRDPFTGIGKPEQLKYGAQGAFSRRITDEHRLVYLVSGEDLVILQA
RYHY

Nucleotide

• Length: 255 bp

>T66443 NZ_CP017297:c546276-546022 [Brachybacterium sp. P6-10-X1]
GTGCGCCTGGTCTGGGACCGCTCCGCCTGGGAGGACTACAAGCACTGGCAGACGGCCGACCGCAAGATTCTCAAGCGGAT
CAACACGCTCATCGACGCCTGCCTGCGCGACCCCTTCACCGGTATCGGCAAGCCCGAACAGCTCAAGTACGGCGCACAGG
GGGCGTTCTCCCGGCGGATCACCGATGAGCACCGTCTCGTCTACCTGGTAAGCGGTGAGGACCTGGTGATCCTCCAGGCG
CGCTACCACTACTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9464.49 Da
• Isoelectric Point: 4.8430

>AT66443 WP_076806325.1 NZ_CP017297:c546528-546277 [Brachybacterium sp. P6-10-X1]
MSISASEARKTLFPLIERVNDDRDAVEIVSRHGNAVLMPAEEYAAWQETAYLFRSPANARRLLEAYERARTGDTQTHDLD
REA

Nucleotide

• Length: 252 bp

>AT66443 NZ_CP017297:c546528-546277 [Brachybacterium sp. P6-10-X1]
ATGTCCATCAGTGCCAGCGAAGCGCGCAAGACGCTGTTTCCGCTCATCGAGCGCGTCAACGACGATCGCGACGCCGTCGA
GATCGTCTCCCGCCACGGGAACGCCGTCCTCATGCCCGCCGAGGAATACGCGGCCTGGCAGGAGACCGCCTACCTGTTCC
GCTCCCCCGCCAACGCCCGCCGACTGCTGGAGGCCTACGAGCGCGCCCGCACCGGCGACACCCAGACGCACGACCTGGAC
CGGGAGGCGTGA