66437

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/RelE-HTH
Location 652422..653026 Replicon chromosome
Accession NZ_CP017296
Organism Pseudomonas fluorescens strain Pt14

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag BH711_RS02985 Protein ID WP_038449428.1
Coordinates 652712..653026 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag BH711_RS02980 Protein ID WP_014717446.1
Coordinates 652422..652709 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BH711_RS02945 647926..648684 + 759 WP_014717370.1 MBL fold metallo-hydrolase -
BH711_RS02950 648712..649425 + 714 WP_005786030.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
BH711_RS02960 649694..649987 - 294 WP_038449426.1 type II toxin-antitoxin system RelE/ParE family toxin -
BH711_RS02965 649977..650216 - 240 WP_014717442.1 type II toxin-antitoxin system RelB/DinJ family antitoxin -
BH711_RS02970 650623..650877 + 255 WP_032903173.1 hypothetical protein -
BH711_RS02975 650951..652177 - 1227 WP_014717445.1 MFS transporter -
BH711_RS02980 652422..652709 - 288 WP_014717446.1 helix-turn-helix domain-containing protein Antitoxin
BH711_RS02985 652712..653026 - 315 WP_038449428.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
BH711_RS02990 653133..654074 - 942 WP_014717448.1 LysR family transcriptional regulator -
BH711_RS02995 654232..655548 + 1317 WP_014717449.1 MHS family MFS transporter -
BH711_RS03000 655560..656792 + 1233 WP_038449430.1 Zn-dependent hydrolase -
BH711_RS03005 656858..657910 + 1053 WP_038449432.1 histone deacetylase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-2

(42-102)

Antitoxin

Cro/C1-type helix-turn-helix domain

(35-86)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11647.39 Da        Isoelectric Point: 10.0575

>T66437 WP_038449428.1 NZ_CP017296:c653026-652712 [Pseudomonas fluorescens]
MIFIETSVFTRRVKELINEDAYTAFQNVLVANPAAGDVIEGTGGIRKIRVAAKSHGKRGGARVIYYHFASASQIVLLMIY
PKNEQQDLSADERKSLKAAIEHWR

Download         Length: 315 bp

>T66437 NZ_CP017296:c653026-652712 [Pseudomonas fluorescens]
ATGATTTTCATTGAGACATCGGTCTTCACACGTCGCGTTAAAGAATTGATCAACGAAGATGCCTACACGGCCTTTCAGAA
CGTGCTTGTAGCGAATCCCGCTGCAGGCGATGTGATTGAGGGTACGGGCGGTATCCGCAAAATACGAGTAGCAGCCAAGA
GCCATGGTAAACGAGGCGGGGCACGTGTGATCTATTACCACTTCGCCTCGGCATCACAGATTGTTTTACTGATGATCTAT
CCCAAAAATGAACAGCAGGACTTGAGCGCCGACGAACGCAAGTCACTGAAGGCTGCGATTGAGCACTGGAGATAG

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10794.39 Da        Isoelectric Point: 7.3219

>AT66437 WP_014717446.1 NZ_CP017296:c652709-652422 [Pseudomonas fluorescens]
MSRLFDELMESVQEMNEILRGEREPSREFHVDSMRVKEIRKATGLTQAKFAALIDVQLGTLRNWEQGRREPTGPAKALLK
AIHNDPVHVLNALAS

Download         Length: 288 bp

>AT66437 NZ_CP017296:c652709-652422 [Pseudomonas fluorescens]
ATGAGCCGACTTTTTGATGAATTGATGGAAAGCGTTCAAGAGATGAACGAGATCCTGCGCGGAGAACGGGAGCCATCCCG
TGAATTTCATGTCGACTCAATGCGAGTTAAAGAGATCCGTAAGGCAACAGGGCTGACCCAGGCTAAATTCGCAGCGCTGA
TTGACGTTCAGCTCGGTACGTTGCGCAACTGGGAGCAGGGTCGTCGCGAACCTACTGGGCCGGCAAAGGCGTTGTTGAAA
GCTATCCATAACGACCCGGTACATGTACTGAACGCGCTGGCTAGCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References