Detailed information of TA system TA066404

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 1392063..1392660
• Replicon: chromosome
• Accession: NZ_CP017289
• Organism: Klebsiella variicola strain GJ3

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A0B7GEF2
• Locus tag: BBD65_RS08925
• Protein ID: WP_012542526.1
• Coordinates: 1392063..1392380 (+)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: BBD65_RS08930
• Protein ID: WP_012542525.1
• Coordinates: 1392373..1392660 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BBD65_RS08905	1387903..1389330	-	1428	WP_023297179.1	MFS transporter	-
BBD65_RS08910	1389439..1390305	+	867	WP_008805530.1	helix-turn-helix transcriptional regulator	-
BBD65_RS08920	1390835..1391878	+	1044	WP_016160437.1	DUF2157 domain-containing protein	-
BBD65_RS08925	1392063..1392380	+	318	WP_012542526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BBD65_RS08930	1392373..1392660	+	288	WP_012542525.1	helix-turn-helix transcriptional regulator	Antitoxin
BBD65_RS08935	1393096..1393749	-	654	WP_012968755.1	oxygen-insensitive NAD(P)H nitroreductase	-
BBD65_RS08940	1393869..1394237	-	369	WP_008805536.1	MmcQ/YjbR family DNA-binding protein	-
BBD65_RS08945	1394237..1394818	-	582	WP_012968754.1	TetR/AcrR family transcriptional regulator	-
BBD65_RS08950	1394998..1396113	+	1116	WP_162493283.1	MBL fold metallo-hydrolase	-
BBD65_RS08955	1396144..1396485	+	342	WP_002893035.1	RamA family antibiotic efflux transcriptional regulator	-
BBD65_RS08960	1396502..1396750	-	249	WP_008805539.1	DUF1158 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-89)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12110.39 Da
• Isoelectric Point: 11.2767

>T66404 WP_012542526.1 NZ_CP017289:1392063-1392380 [Klebsiella variicola]
MFRLVVHVDVKKELQALPPIVQAKMIRQIDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Nucleotide

• Length: 318 bp

>T66404 NZ_CP017289:1392063-1392380 [Klebsiella variicola]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAAATAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10815.51 Da
• Isoelectric Point: 10.2212

>AT66404 WP_012542525.1 NZ_CP017289:1392373-1392660 [Klebsiella variicola]
MSKKIIDWDTLKTELLSQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQILLY

Nucleotide

• Length: 288 bp

>AT66404 NZ_CP017289:1392373-1392660 [Klebsiella variicola]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTAGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTGACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCGACGGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGTGGAATAAAGCACCCACAAATTTTACTTTACTAA

Structures

Toxin

• AlphaFold DB: A0A0B7GEF2