Detailed information of TA system TA066398

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 52151..52420
• Replicon: plasmid pKPGJ-3c
• Accession: NZ_CP017287
• Organism: Klebsiella variicola strain GJ3

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: BBD65_RS01655
• Protein ID: WP_001312861.1
• Coordinates: 52262..52420 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	52151..52216 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BBD65_RS01630	47861..48388	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
BBD65_RS01635	48446..48679	+	234	WP_000006003.1	DUF905 family protein	-
BBD65_RS01640	48740..50763	+	2024	Protein_65	ParB/RepB/Spo0J family partition protein	-
BBD65_RS01645	50832..51266	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
BBD65_RS01650	51263..51982	+	720	WP_001276217.1	plasmid SOS inhibition protein A	-
-	51994..52218	+	225	NuclAT_0	-	-
-	51994..52218	+	225	NuclAT_0	-	-
-	51994..52218	+	225	NuclAT_0	-	-
-	51994..52218	+	225	NuclAT_0	-	-
-	52151..52216	+	66	-	-	Antitoxin
BBD65_RS01655	52262..52420	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
BBD65_RS31400	52658..53035	-	378	Protein_69	hypothetical protein	-
BBD65_RS01675	53335..53631	+	297	WP_001272251.1	hypothetical protein	-
BBD65_RS01680	53742..54563	+	822	WP_001234445.1	DUF945 domain-containing protein	-
BBD65_RS01685	54860..55462	-	603	WP_077143832.1	transglycosylase SLT domain-containing protein	-
BBD65_RS01690	55785..56168	+	384	WP_001354030.1	relaxosome protein TraM	-
BBD65_RS01695	56362..56745	+	384	Protein_74	conjugal transfer protein TrbJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	aph(3')-Ia / mph(A) / blaCTX-M-65 / fosA3 / blaTEM-215	-	1..73385	73385
	-	flank	IS/Tn	-	-	56897..58165	1268

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T66398 WP_001312861.1 NZ_CP017287:52262-52420 [Klebsiella variicola]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T66398 NZ_CP017287:52262-52420 [Klebsiella variicola]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 66 bp

>AT66398 NZ_CP017287:52151-52216 [Klebsiella variicola]
AGAAGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCAC

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin