Detailed information of TA system TA066376

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 39127..39851
• Replicon: plasmid pKPGJ-1b
• Accession: NZ_CP017281
• Organism: Klebsiella variicola strain GJ1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A2J4YLV3
• Locus tag: BBD63_RS00235
• Protein ID: WP_023292165.1
• Coordinates: 39127..39438 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: BBD63_RS00240
• Protein ID: WP_072122726.1
• Coordinates: 39435..39851 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BBD63_RS00215	35135..36037	-	903	WP_060598839.1	LysR family transcriptional regulator	-
BBD63_RS00220	36320..37459	+	1140	WP_072122725.1	PLP-dependent aspartate aminotransferase family protein	-
BBD63_RS00225	37480..38409	+	930	WP_158236771.1	DMT family transporter	-
BBD63_RS00230	38524..38825	+	302	Protein_44	DDE-type integrase/transposase/recombinase	-
BBD63_RS00235	39127..39438	+	312	WP_023292165.1	type II toxin-antitoxin system HigB family toxin	Toxin
BBD63_RS00240	39435..39851	+	417	WP_072122726.1	helix-turn-helix domain-containing protein	Antitoxin
BBD63_RS00245	40668..41048	+	381	WP_077138875.1	hypothetical protein	-
BBD63_RS00250	41114..41461	+	348	WP_077138876.1	hypothetical protein	-
BBD63_RS00255	41549..41779	+	231	WP_004197732.1	hypothetical protein	-
BBD63_RS00260	41826..42659	+	834	WP_077138877.1	type I restriction-modification system subunit M	-
BBD63_RS00265	43284..43814	+	531	WP_077138878.1	antirestriction protein	-
BBD63_RS00270	43852..44331	-	480	WP_077138879.1	transglycosylase SLT domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..92219	92219

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (84-133)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12497.32 Da
• Isoelectric Point: 9.9242

>T66376 WP_023292165.1 NZ_CP017281:39127-39438 [Klebsiella variicola]
VHVISRAPFDEAARHYPNDAAAIDDTYRVLKRVVAKKPDELKRYFTSLDRMKYREKWWVIDIGGNNLRMMFFADFERGKI
FVKHITTHAEYDRLTKYYREHKE

Nucleotide

• Length: 312 bp

>T66376 NZ_CP017281:39127-39438 [Klebsiella variicola]
GTGCATGTTATATCACGAGCCCCTTTTGATGAGGCGGCAAGACACTATCCGAATGATGCAGCGGCCATCGATGACACCTA
TCGTGTACTAAAGCGGGTTGTCGCAAAAAAACCGGATGAGCTAAAGCGATACTTTACTAGTCTGGATCGCATGAAATACC
GGGAGAAATGGTGGGTTATCGATATCGGCGGTAATAACCTTAGAATGATGTTTTTTGCTGATTTTGAGCGCGGAAAGATA
TTCGTCAAACACATAACCACCCATGCTGAATATGATCGCTTAACGAAATATTATCGGGAGCACAAAGAATGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15468.55 Da
• Isoelectric Point: 4.4711

>AT66376 WP_072122726.1 NZ_CP017281:39435-39851 [Klebsiella variicola]
MIFSDAIKAANDLASIVPLLGGSSSRKDYEEALKLVEYLLEHDPDSPLVDMLTARIDTWEDNAVEFEEFNTRIEAGKNGV
SLLRVLMQQHGLSQSDFENEIGKKSLVSRILSGERSLTLDHMRALANRFQIPVSMFVD

Nucleotide

• Length: 417 bp

>AT66376 NZ_CP017281:39435-39851 [Klebsiella variicola]
ATGATTTTTAGCGATGCCATTAAAGCTGCGAATGACCTGGCCAGTATTGTCCCTCTGCTGGGTGGCAGCTCGTCCCGCAA
AGATTACGAAGAGGCACTTAAACTCGTTGAATATCTTCTGGAACATGATCCGGACAGCCCGCTGGTTGATATGCTGACTG
CCCGCATCGATACCTGGGAAGATAACGCGGTGGAATTTGAAGAATTTAATACGCGCATCGAAGCAGGCAAAAACGGTGTA
TCGTTGCTGCGGGTTCTTATGCAGCAACACGGGCTTTCACAGTCCGATTTCGAAAATGAGATAGGTAAAAAATCGCTCGT
CAGCCGGATCCTCAGTGGTGAACGCAGTCTGACCCTTGATCACATGCGGGCGCTGGCAAATCGATTTCAAATCCCGGTCT
CGATGTTTGTTGACTGA

Structures

Toxin

• AlphaFold DB: A0A2J4YLV3