Detailed information of TA system TA066355

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1236200..1236797
• Replicon: chromosome
• Accession: NZ_CP017257
• Organism: Neisseria meningitidis strain COL-201504-11

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A0Y6JZK6
• Locus tag: BFO62_RS07095
• Protein ID: WP_002242826.1
• Coordinates: 1236200..1236478 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: BFO62_RS07100
• Protein ID: WP_002221020.1
• Coordinates: 1236486..1236797 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BFO62_RS07055	1231642..1232313	+	672	WP_002217215.1	CsgG/HfaB family protein	-
BFO62_RS07060	1232323..1232691	+	369	WP_002219297.1	DUF4810 domain-containing protein	-
BFO62_RS07065	1232688..1233335	+	648	WP_002217218.1	DUF799 domain-containing protein	-
BFO62_RS07075	1233777..1234919	+	1143	WP_002221015.1	ABC transporter ATP-binding protein/permease	-
BFO62_RS07080	1235029..1235238	-	210	WP_002221016.1	hypothetical protein	-
BFO62_RS07085	1235235..1235531	-	297	WP_002221017.1	hypothetical protein	-
BFO62_RS07090	1235510..1236115	-	606	WP_076612565.1	hypothetical protein	-
BFO62_RS07095	1236200..1236478	+	279	WP_002242826.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BFO62_RS07100	1236486..1236797	+	312	WP_002221020.1	HigA family addiction module antidote protein	Antitoxin
BFO62_RS07115	1237614..1239587	-	1974	WP_002221022.1	pyocin knob domain-containing protein	-
BFO62_RS07120	1239598..1240158	-	561	WP_002213590.1	YmfQ family protein	-
BFO62_RS07125	1240155..1240766	-	612	WP_002224558.1	baseplate J/gp47 family protein	-
BFO62_RS13100	1240766..1240930	-	165	WP_002224559.1	baseplate J/gp47 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1240995..1241960	965

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (3-80)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10237.66 Da
• Isoelectric Point: 10.0980

>T66355 WP_002242826.1 NZ_CP017257:1236200-1236478 [Neisseria meningitidis]
MIVSFKHKGLERFFKTGSLSGIQAGHSVKLNLLLTALNAAQTPSDMAVPSWNLHPLKGSLSGHWAVKVNGNWRLTFRFND
GNAEVVDYQDYH

Nucleotide

• Length: 279 bp

>T66355 NZ_CP017257:1236200-1236478 [Neisseria meningitidis]
ATGATTGTTTCTTTCAAACACAAAGGGCTTGAACGCTTTTTTAAAACAGGCTCGCTATCGGGCATCCAAGCCGGGCATTC
CGTCAAACTGAATCTGCTTCTAACCGCGCTGAATGCCGCGCAAACGCCTTCGGATATGGCTGTGCCGAGTTGGAATCTTC
ATCCCCTCAAGGGCAGTTTGTCGGGACATTGGGCGGTTAAAGTCAACGGAAATTGGCGTTTGACTTTTCGTTTCAACGAC
GGCAATGCCGAAGTTGTCGATTATCAGGATTATCACTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11476.32 Da
• Isoelectric Point: 10.5523

>AT66355 WP_002221020.1 NZ_CP017257:1236486-1236797 [Neisseria meningitidis]
MKMHNPAHPGLVLKEYINGASITDIAKRLGVSRVALSRIVNAQSSITPEMAVRLSQLLNTSPDFWLNMQAGYDLWQIRQR
KTFDIAPLFPEPPLSSMQTGARA

Nucleotide

• Length: 312 bp

>AT66355 NZ_CP017257:1236486-1236797 [Neisseria meningitidis]
ATGAAAATGCACAATCCTGCCCACCCGGGCCTTGTTTTAAAAGAGTATATTAACGGTGCAAGCATTACCGATATAGCGAA
ACGCCTGGGTGTCAGCCGGGTTGCCTTATCGCGCATTGTCAACGCCCAATCTTCGATTACGCCCGAGATGGCGGTTCGTT
TAAGCCAACTGCTCAATACTTCGCCTGATTTTTGGCTGAATATGCAGGCCGGTTATGATTTGTGGCAAATCAGGCAACGC
AAAACTTTTGATATTGCGCCCTTATTTCCCGAACCGCCTTTGTCTTCGATGCAAACAGGTGCAAGGGCTTAA

Structures

Toxin

• AlphaFold DB: A0A0Y6JZK6