Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 4363912..4364451 Replicon chromosome
Accession NZ_CP017251
Organism Escherichia coli strain NADC 5570/86-24/6564 isolate wild

Toxin (Protein)


Gene name yafQ Uniprot ID -
Locus tag BHW77_RS22650 Protein ID WP_001303998.1
Coordinates 4364191..4364451 (+) Length 87 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID C3TP07
Locus tag BHW77_RS22645 Protein ID WP_000729705.1
Coordinates 4363912..4364172 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BHW77_RS22625 4359666..4360451 - 786 WP_000207556.1 putative lateral flagellar export/assembly protein LafU -
BHW77_RS22630 4360423..4362135 + 1713 Protein_4304 flagellar biosynthesis protein FlhA -
BHW77_RS31465 4362280..4362777 - 498 Protein_4305 REP-associated tyrosine transposase RayT -
BHW77_RS22640 4362953..4363726 - 774 WP_000543891.1 C40 family peptidase -
BHW77_RS22645 4363912..4364172 + 261 WP_000729705.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
BHW77_RS22650 4364191..4364451 + 261 WP_001303998.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
BHW77_RS22655 4364607..4365347 + 741 WP_001225679.1 murein L,D-transpeptidase -
BHW77_RS22660 4365318..4366085 - 768 WP_001301698.1 class II glutamine amidotransferase -
BHW77_RS22665 4366190..4366768 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(3-85)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 87 a.a.        Molecular weight: 10026.71 Da        Isoelectric Point: 10.3188

>T66346 WP_001303998.1 NZ_CP017251:4364191-4364451 [Escherichia coli]
MNSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLRFERTGT
HAALFG

Download         Length: 261 bp

>T66346 NZ_CP017251:4364191-4364451 [Escherichia coli]
TTGAACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAATAAATTGAAATATCTTAT
GACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAGGTTCATGGAAAGGTTATC
GCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGATTTGAGAGAACTGGAACT
CACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9377.74 Da        Isoelectric Point: 5.0758

>AT66346 WP_000729705.1 NZ_CP017251:4363912-4364172 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGV

Download         Length: 261 bp

>AT66346 NZ_CP017251:4363912-4364172 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References