Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type I Classification (family/domain) symER/SymE(toxin)
Location 4709439..4709851 Replicon chromosome
Accession NZ_CP017249
Organism Escherichia coli strain NADC 5570/86-24/6565 isolate mutant

Toxin (Protein)


Gene name symE Uniprot ID Q8FA88
Locus tag BHW76_RS24300 Protein ID WP_000132630.1
Coordinates 4709510..4709851 (+) Length 114 a.a.

Antitoxin (RNA)


Gene name symR
Locus tag -
Coordinates 4709439..4709515 (-)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BHW76_RS24290 4706066..4707535 + 1470 WP_001302468.1 type I restriction-modification system subunit M -
BHW76_RS24295 4707535..4709289 + 1755 WP_000110082.1 restriction endonuclease subunit S -
- 4709439..4709515 - 77 NuclAT_15 - Antitoxin
- 4709439..4709515 - 77 NuclAT_15 - Antitoxin
- 4709439..4709515 - 77 NuclAT_15 - Antitoxin
- 4709439..4709515 - 77 NuclAT_15 - Antitoxin
- 4709439..4709515 - 77 NuclAT_16 - Antitoxin
- 4709439..4709515 - 77 NuclAT_16 - Antitoxin
- 4709439..4709515 - 77 NuclAT_16 - Antitoxin
- 4709439..4709515 - 77 NuclAT_16 - Antitoxin
BHW76_RS24300 4709510..4709851 + 342 WP_000132630.1 endoribonuclease SymE Toxin
BHW76_RS30585 4709898..4711061 - 1164 WP_001304006.1 DUF1524 domain-containing protein -
BHW76_RS24310 4711109..4711990 - 882 WP_001304007.1 DUF262 domain-containing protein -
BHW76_RS24315 4712087..4712251 - 165 WP_000394274.1 DUF1127 domain-containing protein -
BHW76_RS24320 4712428..4713840 + 1413 WP_000199295.1 PLP-dependent aminotransferase family protein -
BHW76_RS32285 4714083..4714283 - 201 WP_001310455.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - espX6 4701638..4722865 21227


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin SymE-like

(20-71)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12294.13 Da        Isoelectric Point: 8.4982

>T66316 WP_000132630.1 NZ_CP017249:4709510-4709851 [Escherichia coli]
MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKGQWLEAAGFATGTVVDVKVMEGCIVLTAQPPAAAESE
LMQSLRQVCKLSARKQRQVQEFIGVIAGKQKVA

Download         Length: 342 bp

>T66316 NZ_CP017249:4709510-4709851 [Escherichia coli]
ATGACTGATACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCAATTAACCGTGAGTTA
TGCGAGTCGTTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CCGGCACGGTGGTAGATGTCAAAGTGATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCGCCGCCGAGAGCGAA
CTGATGCAGTCGCTGCGCCAGGTGTGCAAGCTGTCGGCGCGTAAACAAAGGCAGGTGCAGGAGTTTATTGGGGTGATTGC
GGGTAAACAGAAAGTTGCCTGA

Antitoxin


Download         Length: 77 bp

>AT66316 NZ_CP017249:c4709515-4709439 [Escherichia coli]
AGTCATAACTGCTATTCCCTATAAATAGTGATTGTGATTAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y5T0

Antitoxin

Download structure file

References