Detailed information of TA system TA066313

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4358080..4358774
• Replicon: chromosome
• Accession: NZ_CP017249
• Organism: Escherichia coli strain NADC 5570/86-24/6565 isolate mutant

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: C3TNX7
• Locus tag: BHW76_RS22620
• Protein ID: WP_001263495.1
• Coordinates: 4358080..4358478 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: BHW76_RS22625
• Protein ID: WP_000554757.1
• Coordinates: 4358481..4358774 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
-	4353667..4353747	-	81	NuclAT_12	-	-
-	4353667..4353747	-	81	NuclAT_12	-	-
-	4353667..4353747	-	81	NuclAT_12	-	-
-	4353667..4353747	-	81	NuclAT_12	-	-
BHW76_RS22595	4354343..4354801	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
BHW76_RS22600	4355062..4356519	+	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
BHW76_RS22605	4356576..4357097	-	522	Protein_4302	peptide chain release factor H	-
BHW76_RS22610	4357096..4357299	-	204	Protein_4303	RNA ligase RtcB family protein	-
BHW76_RS22615	4357618..4358070	-	453	WP_001059871.1	GNAT family N-acetyltransferase	-
BHW76_RS22620	4358080..4358478	-	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
BHW76_RS22625	4358481..4358774	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
BHW76_RS22630	4358826..4359881	-	1056	WP_001226188.1	DNA polymerase IV	-
BHW76_RS22635	4359952..4360737	-	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
BHW76_RS22640	4360709..4362421	+	1713	Protein_4309	flagellar biosynthesis protein FlhA	-
BHW76_RS31465	4362566..4363063	-	498	Protein_4310	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4321245..4359881	38636

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.86 Da
• Isoelectric Point: 8.0949

>T66313 WP_001263495.1 NZ_CP017249:c4358478-4358080 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T66313 NZ_CP017249:c4358478-4358080 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAGCTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACTTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAATCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT66313 WP_000554757.1 NZ_CP017249:c4358774-4358481 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT66313 NZ_CP017249:c4358774-4358481 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAATTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: S1FJN6