Detailed information of TA system TA066250

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 101840..102073
• Replicon: plasmid pFAM21845_1
• Accession: NZ_CP017221
• Organism: Escherichia coli strain FAM21845

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: BHT24_RS26465
• Protein ID: WP_001312861.1
• Coordinates: 101840..101998 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	102042..102073 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BHT24_RS26420	97213..97902	-	690	WP_000283383.1	conjugal transfer transcriptional regulator TraJ	-
BHT24_RS26425	98089..98472	-	384	WP_001151528.1	relaxosome protein TraM	-
BHT24_RS26430	98793..99395	+	603	WP_000243710.1	transglycosylase SLT domain-containing protein	-
BHT24_RS26435	99692..100513	-	822	WP_001234423.1	DUF945 domain-containing protein	-
BHT24_RS26440	100633..100830	-	198	WP_052318758.1	hypothetical protein	-
BHT24_RS26450	101186..101398	-	213	WP_001208963.1	hypothetical protein	-
BHT24_RS26465	101840..101998	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	102042..102073	-	32	NuclAT_1	-	Antitoxin
-	102042..102073	-	32	NuclAT_1	-	Antitoxin
-	102042..102073	-	32	NuclAT_1	-	Antitoxin
-	102042..102073	-	32	NuclAT_1	-	Antitoxin
-	103515..103712	-	198	NuclAT_0	-	-
-	103515..103712	-	198	NuclAT_0	-	-
-	103515..103712	-	198	NuclAT_0	-	-
-	103515..103712	-	198	NuclAT_0	-	-
BHT24_RS27940	103524..103712	+	189	WP_001299721.1	hypothetical protein	-
BHT24_RS26475	103724..104443	-	720	WP_081178411.1	plasmid SOS inhibition protein A	-
BHT24_RS26480	104440..104874	-	435	WP_000845932.1	conjugation system SOS inhibitor PsiB	-
BHT24_RS26490	104929..106887	-	1959	Protein_122	ParB/RepB/Spo0J family partition protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(B) / aph(3'')-Ib / aph(6)-Id / aph(3')-Ia / aph(4)-Ia / aac(3)-IVa / dfrA1 / ant(3'')-Ia / qacE / sul1 / blaTEM-1A / sitABCD	iutA / iucD / iucC / iucB / iucA	1..147225	147225

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T66250 WP_001312861.1 NZ_CP017221:c101998-101840 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T66250 NZ_CP017221:c101998-101840 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 32 bp

>AT66250 NZ_CP017221:c102073-102042 [Escherichia coli]
CACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin