Detailed information of TA system TA066225

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 288346..289040
• Replicon: chromosome
• Accession: NZ_CP017220
• Organism: Escherichia coli strain FAM21845

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: BHT24_RS01450
• Protein ID: WP_001263493.1
• Coordinates: 288642..289040 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: BHT24_RS01445
• Protein ID: WP_000554757.1
• Coordinates: 288346..288639 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BHT24_RS01420	284163..284630	+	468	WP_000725257.1	flagellar basal body-associated FliL family protein	-
BHT24_RS01425	284650..285366	+	717	WP_000938723.1	FliA/WhiG family RNA polymerase sigma factor	-
BHT24_RS01430	285379..286242	+	864	WP_001169518.1	flagellar motor stator protein MotA	-
BHT24_RS01435	286245..287168	+	924	WP_001232547.1	putative lateral flagellar export/assembly protein LafU	-
BHT24_RS01440	287239..288294	+	1056	WP_001226164.1	DNA polymerase IV	-
BHT24_RS01445	288346..288639	+	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
BHT24_RS01450	288642..289040	+	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
BHT24_RS01455	289050..289502	+	453	WP_001059847.1	GNAT family N-acetyltransferase	-
BHT24_RS01460	289748..289951	+	204	Protein_271	RNA ligase RtcB family protein	-
BHT24_RS01465	289950..290471	+	522	Protein_272	peptide chain release factor H	-
BHT24_RS01470	290528..291985	-	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
BHT24_RS01475	292246..292704	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
BHT24_RS01480	292796..294040	+	1245	WP_044864403.1	esterase FrsA	-
-	293300..293380	+	81	NuclAT_11	-	-
-	293300..293380	+	81	NuclAT_11	-	-
-	293300..293380	+	81	NuclAT_11	-	-
-	293300..293380	+	81	NuclAT_11	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T66225 WP_001263493.1 NZ_CP017220:288642-289040 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T66225 NZ_CP017220:288642-289040 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT66225 WP_000554757.1 NZ_CP017220:288346-288639 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT66225 NZ_CP017220:288346-288639 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6