Detailed information of TA system TA066211

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4464564..4465166
• Replicon: chromosome
• Accession: NZ_CP017186
• Organism: Enterobacter hormaechei subsp. hoffmannii strain DSM 14563

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A155DUZ6
• Locus tag: BFV65_RS21090
• Protein ID: WP_022649837.1
• Coordinates: 4464855..4465166 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: BFV65_RS21085
• Protein ID: WP_032626927.1
• Coordinates: 4464564..4464854 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BFV65_RS21070	4462062..4462964	+	903	WP_003861960.1	formate dehydrogenase subunit beta	-
BFV65_RS21075	4462961..4463596	+	636	WP_006808711.1	formate dehydrogenase cytochrome b556 subunit	-
BFV65_RS21080	4463593..4464522	+	930	WP_069597066.1	formate dehydrogenase accessory protein FdhE	-
BFV65_RS21085	4464564..4464854	-	291	WP_032626927.1	helix-turn-helix domain-containing protein	Antitoxin
BFV65_RS21090	4464855..4465166	-	312	WP_022649837.1	hypothetical protein	Toxin
BFV65_RS21095	4465315..4466256	-	942	WP_022649838.1	fatty acid biosynthesis protein FabY	-
BFV65_RS21100	4466301..4466738	-	438	WP_015569909.1	D-tyrosyl-tRNA(Tyr) deacylase	-
BFV65_RS21105	4466735..4467616	-	882	WP_069597067.1	virulence factor BrkB family protein	-
BFV65_RS21110	4467610..4468209	-	600	WP_003861947.1	glucose-1-phosphatase	-
BFV65_RS21115	4468328..4469128	-	801	WP_023298924.1	DeoR/GlpR family DNA-binding transcription regulator	-
BFV65_RS21120	4469163..4470059	-	897	WP_069597068.1	sugar kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12148.13 Da
• Isoelectric Point: 9.4455

>T66211 WP_022649837.1 NZ_CP017186:c4465166-4464855 [Enterobacter hormaechei subsp. hoffmannii]
MLFIETDIFTEDVKTLLDDDEYHRFQIFLATQPDYGDLIQNTGGLRKIRWLAGGKGKRSGVRVIYFHRTRECEIRLLLIY
RKGIKDDLSAGEKAILKKMIERW

Nucleotide

• Length: 312 bp

>T66211 NZ_CP017186:c4465166-4464855 [Enterobacter hormaechei subsp. hoffmannii]
ATGCTATTCATTGAAACGGATATTTTTACAGAAGACGTCAAAACGTTACTGGATGACGACGAATATCATAGGTTCCAAAT
ATTCCTGGCAACGCAACCGGATTACGGCGATCTGATCCAGAATACGGGAGGCTTGCGAAAGATTCGCTGGCTTGCTGGCG
GTAAGGGAAAGCGTAGTGGAGTCAGAGTTATCTATTTTCACCGTACGCGTGAGTGTGAAATCAGGTTGCTGCTCATCTAT
CGCAAAGGGATCAAAGATGACCTCTCCGCGGGTGAAAAAGCCATACTGAAAAAGATGATTGAGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10685.18 Da
• Isoelectric Point: 10.5888

>AT66211 WP_032626927.1 NZ_CP017186:c4464854-4464564 [Enterobacter hormaechei subsp. hoffmannii]
MDKALFERLTQSIAQINEIAEGQREPSRTFQIDAMKIKEIRQASGLSQSKFANLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVIPALSQ

Nucleotide

• Length: 291 bp

>AT66211 NZ_CP017186:c4464854-4464564 [Enterobacter hormaechei subsp. hoffmannii]
ATGGATAAGGCGCTGTTTGAGCGATTAACCCAAAGCATTGCGCAAATCAACGAAATTGCCGAGGGCCAACGAGAGCCGTC
GCGTACGTTTCAAATTGATGCGATGAAAATAAAAGAAATTCGTCAGGCGTCCGGGTTGTCGCAGAGTAAATTCGCGAACT
TAATCTCAGTTAGCGTTGATACGTTGCGTAACTGGGAACAAGGCAGACGCTCACCTACAGGCCCCGCTAAAGCGCTATTG
CGCGCCATTGCTAACGACCCTCAACATGTCATCCCAGCCCTATCGCAGTAA

Structures

Toxin

• AlphaFold DB: A0A155DUZ6