Detailed information of TA system TA066181

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3558671..3559328
• Replicon: chromosome
• Accession: NZ_CP017183
• Organism: Enterobacter hormaechei subsp. xiangfangensis strain LMG27195

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A837FDC1
• Locus tag: BFV63_RS16925
• Protein ID: WP_015571638.1
• Coordinates: 3558671..3558970 (+)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: BFV63_RS16930
• Protein ID: WP_048209500.1
• Coordinates: 3558981..3559328 (+)
• Length: 116 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BFV63_RS16915	3555048..3557240	+	2193	WP_048210580.1	type I secretion system permease/ATPase	-
BFV63_RS16920	3557260..3558420	+	1161	WP_086581339.1	HlyD family efflux transporter periplasmic adaptor subunit	-
BFV63_RS16925	3558671..3558970	+	300	WP_015571638.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BFV63_RS16930	3558981..3559328	+	348	WP_048209500.1	HigA family addiction module antidote protein	Antitoxin
BFV63_RS16935	3559404..3560585	-	1182	WP_023314694.1	PLP-dependent aminotransferase family protein	-
BFV63_RS16940	3560606..3561493	-	888	WP_048241982.1	LysR family transcriptional regulator	-
BFV63_RS16945	3561591..3562193	+	603	WP_023314696.1	short chain dehydrogenase	-
BFV63_RS16950	3562190..3562921	-	732	WP_023314697.1	class I SAM-dependent methyltransferase	-
BFV63_RS16955	3562996..3563994	-	999	WP_022651706.1	transketolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (25-72)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11624.29 Da
• Isoelectric Point: 9.6776

>T66181 WP_015571638.1 NZ_CP017183:3558671-3558970 [Enterobacter hormaechei subsp. xiangfangensis]
MINHFRDQWLEDFFLYGRSSNVIPLNLETALARKLDIINAAMSHLDLRSPPGNMYEALSPPLKGYSSIRVNRQYRLVFRW
SEGKADDLYLSPHKYTQHK

Nucleotide

• Length: 300 bp

>T66181 NZ_CP017183:3558671-3558970 [Enterobacter hormaechei subsp. xiangfangensis]
ATGATTAATCATTTTCGGGATCAATGGCTTGAGGATTTTTTTCTCTACGGGAGATCGAGCAATGTTATTCCTTTGAATCT
GGAAACAGCGCTTGCAAGAAAGCTCGACATCATCAATGCGGCCATGTCGCACCTGGATTTACGATCGCCACCGGGCAATA
TGTATGAAGCATTGAGTCCTCCGTTGAAGGGATACTCCTCTATCCGGGTAAACAGACAATACAGGCTTGTATTTCGCTGG
TCAGAGGGAAAAGCAGACGATCTCTATCTCTCTCCACACAAGTACACGCAACACAAGTGA

Antitoxin

Protein

• Length: 116 a.a.
• Molecular weight: 13126.18 Da
• Isoelectric Point: 9.5932

>AT66181 WP_048209500.1 NZ_CP017183:3558981-3559328 [Enterobacter hormaechei subsp. xiangfangensis]
MTLQQALRKPTMPGEVLQYEYLEPLNLKINDLAEMLNVHRNTVSALVNNNRKLTADMAIKLAKAFNTTIEFWLNLQLNVD
IWEAQSNSRTQEELSRIKTVAEVMAKRKSGKPDVA

Nucleotide

• Length: 348 bp

>AT66181 NZ_CP017183:3558981-3559328 [Enterobacter hormaechei subsp. xiangfangensis]
ATGACACTTCAACAGGCACTCCGCAAACCCACCATGCCGGGCGAGGTGTTGCAGTATGAGTATCTTGAACCGCTCAATCT
GAAAATCAACGATCTGGCAGAGATGCTAAATGTACACCGCAATACCGTAAGCGCGTTGGTCAATAACAATCGCAAACTTA
CTGCCGATATGGCGATCAAACTGGCAAAAGCCTTTAATACCACTATTGAATTTTGGCTGAACTTACAGCTAAACGTTGAT
ATCTGGGAAGCGCAATCTAACTCCCGCACGCAGGAGGAGTTAAGCCGGATAAAAACCGTTGCGGAAGTCATGGCGAAGCG
AAAATCCGGCAAGCCGGACGTCGCCTGA

Structures

Toxin

• AlphaFold DB: A0A837FDC1