Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 32083..32813 Replicon chromosome
Accession NZ_CP017181
Organism Enterobacter kobei strain DSM 13645

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag BFV64_RS00155 Protein ID WP_045134487.1
Coordinates 32083..32397 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag BFV64_RS00160 Protein ID WP_023331687.1
Coordinates 32394..32813 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BFV64_RS00130 27749..28837 + 1089 WP_032635252.1 cellulase family glycosylhydrolase -
BFV64_RS00135 28740..29924 - 1185 WP_032637792.1 multidrug efflux MFS transporter EmrD -
BFV64_RS00140 30100..30933 - 834 WP_045134488.1 DMT family transporter -
BFV64_RS00145 30996..31442 - 447 WP_014881927.1 GNAT family N-acetyltransferase -
BFV64_RS23855 31507..31596 - 90 WP_045281349.1 type I toxin-antitoxin system toxin TisB -
BFV64_RS00155 32083..32397 + 315 WP_045134487.1 type II toxin-antitoxin system HigB family toxin Toxin
BFV64_RS00160 32394..32813 + 420 WP_023331687.1 helix-turn-helix domain-containing protein Antitoxin
BFV64_RS25170 32963..33061 + 99 WP_023331688.1 ilvB operon leader peptide IvbL -
BFV64_RS00165 33168..34856 + 1689 WP_023331689.1 acetolactate synthase large subunit -
BFV64_RS00170 34860..35147 + 288 WP_010426528.1 acetolactate synthase small subunit -
BFV64_RS00175 35230..35823 + 594 WP_014881931.1 transcriptional regulator UhpA -
BFV64_RS00180 35820..37325 + 1506 WP_069601594.1 signal transduction histidine-protein kinase/phosphatase UhpB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(21-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-136)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12455.66 Da        Isoelectric Point: 10.3843

>T66153 WP_045134487.1 NZ_CP017181:32083-32397 [Enterobacter kobei]
MHLISMKAILDAVIQFPQHREELLFLGRTIEKSHCTTPVALRKIFPTLDNLKYLDKHYVIDIANNKLRVVALIFFESQKF
YVRHVFTHKEYDRFTEKHRTKGKK

Download         Length: 315 bp

>T66153 NZ_CP017181:32083-32397 [Enterobacter kobei]
ATGCATTTAATCTCAATGAAGGCAATTTTGGATGCGGTGATCCAGTTTCCGCAACACAGAGAGGAGCTTCTCTTTCTGGG
GCGAACCATCGAAAAGAGCCATTGCACGACGCCTGTTGCGCTAAGAAAAATCTTTCCAACGCTAGATAATTTGAAGTATC
TCGATAAGCATTATGTTATTGATATTGCTAACAACAAACTCAGAGTAGTGGCTCTCATATTCTTTGAAAGCCAAAAGTTT
TATGTGCGCCATGTTTTTACTCATAAGGAATATGACCGTTTTACGGAGAAACATCGCACTAAGGGGAAGAAATGA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15561.18 Da        Isoelectric Point: 5.6761

>AT66153 WP_023331687.1 NZ_CP017181:32394-32813 [Enterobacter kobei]
MMIVADAMKATHALVAAVPLLGEHPNEKDYQDALELVEYLLMNEPGSPLLDIVCARIRRYEANRPEIVALRQEMESVPVG
IAVLRTLMDQYKLTISDFQDEIGSKSMVSRVLNGQRQLTLNHIKKLAARFGVSPALFIE

Download         Length: 420 bp

>AT66153 NZ_CP017181:32394-32813 [Enterobacter kobei]
ATGATGATCGTAGCTGATGCAATGAAAGCAACGCATGCCCTTGTCGCGGCGGTTCCTTTATTGGGTGAACATCCAAACGA
GAAAGATTACCAGGACGCACTTGAGCTGGTTGAGTATTTACTGATGAACGAACCTGGCAGCCCTTTGCTGGATATCGTAT
GTGCAAGAATTCGTCGTTACGAAGCCAATCGGCCCGAAATTGTCGCCTTACGCCAGGAGATGGAATCTGTCCCAGTGGGC
ATTGCCGTTTTAAGAACCCTTATGGATCAATACAAACTGACTATTTCGGATTTTCAGGATGAAATTGGCAGTAAATCAAT
GGTTTCAAGGGTGTTGAACGGTCAACGTCAGCTGACGTTGAACCATATAAAAAAACTGGCTGCTCGCTTTGGGGTATCGC
CTGCGCTGTTCATTGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References