Detailed information of TA system TA066143

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 2556356..2557016
• Replicon: chromosome
• Accession: NZ_CP017180
• Organism: Enterobacter hormaechei subsp. oharae strain DSM 16687

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: BFV66_RS12270
• Protein ID: WP_047053529.1
• Coordinates: 2556356..2556709 (+)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A2J0Q4Z7
• Locus tag: BFV66_RS12275
• Protein ID: WP_017383313.1
• Coordinates: 2556714..2557016 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BFV66_RS12250	2552789..2553712	+	924	WP_047053526.1	L,D-transpeptidase	-
BFV66_RS12260	2554201..2555118	+	918	WP_003859531.1	nitrogen assimilation transcriptional regulator NAC	-
BFV66_RS12265	2555215..2556165	+	951	WP_003859532.1	HTH-type transcriptional regulator Cbl	-
BFV66_RS12270	2556356..2556709	+	354	WP_047053529.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BFV66_RS12275	2556714..2557016	+	303	WP_017383313.1	helix-turn-helix transcriptional regulator	Antitoxin
BFV66_RS12285	2557305..2559494	-	2190	WP_047053531.1	TonB-dependent siderophore receptor	-
BFV66_RS12290	2559521..2560846	-	1326	WP_047053533.1	SidA/IucD/PvdA family monooxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• HigA2-like, helix-turn-helix domain (18-89)

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 13522.40 Da
• Isoelectric Point: 10.1729

>T66143 WP_047053529.1 NZ_CP017180:2556356-2556709 [Enterobacter hormaechei subsp. oharae]
VWAIKTTDRFDDWFTSLNDSERASVLAALLVLREKGPGLSRPYADTLKGSRHSNMKELRIQSKGDPLRAFFAFNPNRTGI
VLCAGNKIGNERRFYDEMLLVADREYTRWLNTLKERN

Nucleotide

• Length: 354 bp

>T66143 NZ_CP017180:2556356-2556709 [Enterobacter hormaechei subsp. oharae]
GTGTGGGCGATTAAAACTACGGACAGGTTTGACGACTGGTTCACCTCATTAAACGATAGCGAGAGAGCCAGCGTACTGGC
CGCGCTACTTGTATTGCGGGAAAAGGGACCGGGTCTGTCCAGACCGTATGCCGATACGCTGAAAGGCTCCCGCCACAGCA
ACATGAAAGAATTGCGTATCCAGAGTAAGGGTGATCCGCTGAGGGCATTTTTCGCTTTCAATCCGAACCGTACGGGTATT
GTACTGTGTGCGGGCAATAAGATCGGTAATGAAAGGCGATTTTACGATGAGATGCTCCTCGTTGCGGATCGGGAATACAC
TCGCTGGCTGAACACATTAAAAGAGAGGAATTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11028.85 Da
• Isoelectric Point: 6.4727

>AT66143 WP_017383313.1 NZ_CP017180:2556714-2557016 [Enterobacter hormaechei subsp. oharae]
MGRTLEQLIADEKPEVVASAQAMATDILLNIHLAELREKVQKTQVDMARALGIKQPTVAVMEKAGRDIKLSSLKRYVEAA
GGKLRLDVELPDGSHYEFVL

Nucleotide

• Length: 303 bp

>AT66143 NZ_CP017180:2556714-2557016 [Enterobacter hormaechei subsp. oharae]
ATGGGCAGAACCCTGGAGCAGCTTATTGCAGATGAAAAACCTGAGGTTGTTGCCAGCGCACAGGCAATGGCAACGGATAT
TTTGCTCAATATCCATCTGGCCGAGTTACGTGAAAAAGTGCAAAAAACGCAGGTAGATATGGCCAGGGCGTTGGGTATTA
AACAACCTACTGTCGCCGTCATGGAAAAGGCAGGGCGCGATATTAAGCTTTCTTCTTTGAAACGCTACGTTGAGGCCGCC
GGAGGGAAGCTACGGCTGGATGTAGAACTGCCCGATGGTTCTCATTACGAGTTTGTGTTGTAA

Structures

Antitoxin

• AlphaFold DB: A0A2J0Q4Z7