Detailed information of TA system TA066135

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 66434..66936
• Replicon: chromosome
• Accession: NZ_CP017180
• Organism: Enterobacter hormaechei subsp. oharae strain DSM 16687

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: -
• Locus tag: BFV66_RS00340
• Protein ID: WP_047050704.1
• Coordinates: 66434..66688 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: -
• Locus tag: BFV66_RS00345
• Protein ID: WP_047050702.1
• Coordinates: 66685..66936 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BFV66_RS00315	61898..62422	-	525	WP_047050709.1	lipocalin family protein	-
BFV66_RS00320	62540..63265	+	726	WP_017383646.1	MerR family transcriptional regulator	-
BFV66_RS00325	63365..63775	+	411	WP_022650126.1	hydroxyisourate hydrolase	-
BFV66_RS00330	63878..64837	+	960	WP_047050707.1	DUF523 and DUF1722 domain-containing protein	-
BFV66_RS00335	64984..66072	+	1089	WP_047050706.1	AAA family ATPase	-
BFV66_RS23985	66203..66358	-	156	WP_154815872.1	hypothetical protein	-
BFV66_RS00340	66434..66688	-	255	WP_047050704.1	Txe/YoeB family addiction module toxin	Toxin
BFV66_RS00345	66685..66936	-	252	WP_047050702.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
BFV66_RS00350	67187..68437	-	1251	WP_047050701.1	HlyD family efflux transporter periplasmic adaptor subunit	-
BFV66_RS00355	68444..69193	-	750	WP_047050700.1	hypothetical protein	-
BFV66_RS00360	69190..71286	-	2097	WP_047050699.1	peptidase domain-containing ABC transporter	-
BFV66_RS00365	71354..71539	-	186	WP_063159820.1	hypothetical protein	-
BFV66_RS00370	71563..71757	-	195	WP_000644465.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	45379..66936	21557

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (2-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10117.66 Da
• Isoelectric Point: 7.8747

>T66135 WP_047050704.1 NZ_CP017180:c66688-66434 [Enterobacter hormaechei subsp. oharae]
VKITWSEEAWEDYLYWQETDKRMVRKINELIKISCRTPFEGIGKPEPLKHNLAGFWSRRITSEHRMVYAITEEAILIAAC
RYHY

Nucleotide

• Length: 255 bp

>T66135 NZ_CP017180:c66688-66434 [Enterobacter hormaechei subsp. oharae]
GTGAAGATCACATGGTCTGAAGAGGCATGGGAAGACTACCTCTATTGGCAGGAGACCGATAAGCGCATGGTGAGGAAAAT
CAATGAGCTTATCAAAATCTCCTGTCGCACTCCGTTTGAGGGGATCGGCAAGCCAGAGCCATTAAAACATAACCTGGCTG
GCTTCTGGTCTCGCCGAATCACCTCTGAGCATCGCATGGTCTATGCCATCACAGAAGAAGCGATCCTCATAGCTGCGTGC
CGATATCACTACTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9343.57 Da
• Isoelectric Point: 4.7854

>AT66135 WP_047050702.1 NZ_CP017180:c66936-66685 [Enterobacter hormaechei subsp. oharae]
MRVISYSEARQNLSATMVQTTEDRAPVLITRQNGESCVLMSLEEYNSLEETAYLMRSPANAKRLMNSIESLKSGSGKERD
IIE

Nucleotide

• Length: 252 bp

>AT66135 NZ_CP017180:c66936-66685 [Enterobacter hormaechei subsp. oharae]
ATGCGTGTTATTAGTTACAGTGAAGCCCGACAAAACCTGTCGGCAACCATGGTTCAGACGACGGAAGACCGCGCGCCGGT
GCTTATTACGCGCCAGAACGGTGAATCTTGTGTTCTGATGTCGCTGGAAGAGTACAACTCGCTTGAAGAAACCGCATACC
TGATGCGCTCCCCGGCAAACGCAAAACGTCTGATGAACTCTATCGAGAGCTTGAAATCTGGTAGCGGGAAGGAAAGGGAC
ATTATTGAGTGA