Detailed information of TA system TA066134

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 51923..52569
• Replicon: chromosome
• Accession: NZ_CP017180
• Organism: Enterobacter hormaechei subsp. oharae strain DSM 16687

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: BFV66_RS00260
• Protein ID: WP_047050720.1
• Coordinates: 51923..52273 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A7H8UAV4
• Locus tag: BFV66_RS00265
• Protein ID: WP_045335986.1
• Coordinates: 52270..52569 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BFV66_RS00235	47600..48070	+	471	WP_015703840.1	DNA repair protein RadC	-
BFV66_RS00240	48083..48409	+	327	WP_059443453.1	type IV toxin-antitoxin system YeeU family antitoxin	-
BFV66_RS00245	48430..48750	+	321	WP_059443702.1	TA system toxin CbtA family protein	-
BFV66_RS00250	49045..50601	+	1557	WP_047050722.1	hypothetical protein	-
BFV66_RS22740	50641..50817	-	177	WP_071962261.1	toxin-antitoxin system HicB family antitoxin	-
BFV66_RS00255	50954..51295	+	342	WP_006812446.1	SymE family type I addiction module toxin	-
BFV66_RS23495	51398..51650	+	253	Protein_54	DNA-binding protein	-
BFV66_RS00260	51923..52273	+	351	WP_047050720.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BFV66_RS00265	52270..52569	+	300	WP_045335986.1	XRE family transcriptional regulator	Antitoxin
BFV66_RS00270	52634..53515	-	882	WP_047050719.1	helix-turn-helix domain-containing protein	-
BFV66_RS00275	53758..54237	-	480	WP_047050718.1	hypothetical protein	-
BFV66_RS00280	54239..55027	-	789	WP_045329485.1	winged helix-turn-helix domain-containing protein	-
BFV66_RS00285	55861..56463	+	603	WP_047050717.1	response regulator transcription factor	-
BFV66_RS00290	56516..57100	+	585	WP_023299002.1	type 1 fimbrial protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	45379..66936	21557

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-105)

Antitoxin

• HigA2-like, helix-turn-helix domain (12-88)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13497.59 Da
• Isoelectric Point: 8.8416

>T66134 WP_047050720.1 NZ_CP017180:51923-52273 [Enterobacter hormaechei subsp. oharae]
MWTVLFSKVFEQWLLEQEDGLQEKVLADLLNLQNYGPRLPRPYADTVKGSQYKNMKELRIQYAGRPVRAFFAFDPVRQAI
VLCAGDKRNDKTFYEKLIRIADAEFTLHLTSLEAAK

Nucleotide

• Length: 351 bp

>T66134 NZ_CP017180:51923-52273 [Enterobacter hormaechei subsp. oharae]
ATGTGGACGGTACTGTTTAGTAAGGTATTCGAGCAATGGTTGCTTGAACAGGAAGATGGGTTGCAGGAAAAGGTATTGGC
AGACCTGTTAAATCTACAGAATTATGGACCGCGTCTTCCTCGTCCCTATGCGGATACCGTTAAAGGGTCACAGTATAAGA
ATATGAAAGAGTTACGTATTCAATATGCGGGTCGCCCGGTTCGTGCGTTCTTCGCGTTTGATCCTGTACGTCAGGCCATT
GTGCTTTGCGCGGGCGATAAGCGTAATGACAAAACCTTTTATGAAAAACTGATTCGCATTGCAGATGCCGAATTCACCCT
TCATCTGACAAGCCTGGAGGCAGCCAAGTGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11129.87 Da
• Isoelectric Point: 6.4714

>AT66134 WP_045335986.1 NZ_CP017180:52270-52569 [Enterobacter hormaechei subsp. oharae]
VKTLNEVIASRSPESQQRIQEMAEEMFLETGLQLLREELQLSQKHVADLMGVSQPAVTQLEQRGNDLKIATLKRYIEAMG
GKLSINVELPTGKRVAFHL

Nucleotide

• Length: 300 bp

>AT66134 NZ_CP017180:52270-52569 [Enterobacter hormaechei subsp. oharae]
GTGAAAACCTTAAATGAAGTGATTGCGTCACGCTCACCGGAAAGCCAACAGCGCATTCAGGAGATGGCAGAGGAAATGTT
CCTCGAAACCGGTCTGCAACTTCTGCGTGAAGAGCTCCAGCTTTCGCAAAAACATGTCGCCGATCTTATGGGCGTAAGTC
AACCTGCGGTAACACAGCTGGAGCAGCGTGGAAACGATCTTAAGATTGCTACGCTTAAGCGTTACATCGAAGCGATGGGC
GGAAAACTTAGCATTAACGTGGAACTTCCCACTGGCAAGCGGGTTGCGTTTCATCTCTAG

Structures

Antitoxin

• AlphaFold DB: A0A7H8UAV4