Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1196396..1197123 Replicon chromosome
Accession NZ_CP017179
Organism Enterobacter hormaechei subsp. steigerwaltii strain DSM 16691

Toxin (Protein)


Gene name higB Uniprot ID A0A2J0Q486
Locus tag BFV68_RS05610 Protein ID WP_033486526.1
Coordinates 1196812..1197123 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A2J0Q1H0
Locus tag BFV68_RS05605 Protein ID WP_045349893.1
Coordinates 1196396..1196815 (-) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BFV68_RS05595 1194226..1195347 - 1122 WP_015571201.1 glutamate--cysteine ligase -
BFV68_RS05600 1195453..1196343 + 891 WP_069595705.1 oxidoreductase -
BFV68_RS05605 1196396..1196815 - 420 WP_045349893.1 helix-turn-helix domain-containing protein Antitoxin
BFV68_RS05610 1196812..1197123 - 312 WP_033486526.1 type II toxin-antitoxin system HigB family toxin Toxin
BFV68_RS05615 1197545..1199665 - 2121 WP_033486523.1 hypothetical protein -
BFV68_RS23645 1200164..1200592 + 429 WP_137984238.1 hypothetical protein -
BFV68_RS05620 1200544..1201854 + 1311 WP_137984239.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(83-134)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12506.30 Da        Isoelectric Point: 9.9435

>T66117 WP_033486526.1 NZ_CP017179:c1197123-1196812 [Enterobacter hormaechei subsp. steigerwaltii]
MHIVSRAPFDTAARHYPNQAQALDDLYRVLKKEHYPSPDEMRKRFPSLDRMKYREKWWVIDVGGGHLRVMFFADFERGKI
FIKHISTHADYDKLTDYYRRTKE

Download         Length: 312 bp

>T66117 NZ_CP017179:c1197123-1196812 [Enterobacter hormaechei subsp. steigerwaltii]
ATGCACATAGTCTCAAGAGCGCCTTTCGACACAGCGGCCAGACATTATCCCAATCAAGCACAAGCCCTGGACGATCTTTA
CCGGGTACTAAAAAAGGAACACTACCCCTCACCAGACGAGATGCGAAAGCGCTTTCCTAGCCTGGACCGGATGAAATACC
GGGAAAAATGGTGGGTCATTGATGTGGGTGGCGGGCATCTAAGAGTGATGTTTTTTGCTGATTTTGAGCGTGGAAAGATC
TTCATCAAACACATCAGCACTCACGCAGACTATGACAAGCTCACGGACTACTACCGGAGAACGAAAGAATGA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15375.45 Da        Isoelectric Point: 4.7132

>AT66117 WP_045349893.1 NZ_CP017179:c1196815-1196396 [Enterobacter hormaechei subsp. steigerwaltii]
MNYAAAIKAANALTNELPFLGSSPSRQDYEDALALVEYLIEHDPDNPLVDMLTAKIDKYENASPEFAEFNARIASIPSGV
ALLRTLMDQYQLTQSDFENEIGKKSLVSRILNGQRTLTLDHMRALAKRFGLPVSAFVGN

Download         Length: 420 bp

>AT66117 NZ_CP017179:c1196815-1196396 [Enterobacter hormaechei subsp. steigerwaltii]
ATGAATTATGCAGCAGCCATCAAAGCGGCAAACGCTCTGACAAACGAGCTCCCTTTCCTGGGTTCAAGCCCGTCGCGGCA
AGACTACGAGGATGCGCTGGCGCTGGTAGAGTATCTTATCGAGCACGACCCCGATAACCCGCTGGTAGATATGCTCACTG
CCAAAATCGATAAGTATGAAAACGCGTCCCCGGAGTTTGCAGAATTTAACGCGCGTATTGCCAGCATTCCGTCAGGCGTT
GCGTTACTGCGAACGCTAATGGATCAGTACCAGCTCACTCAGAGTGATTTCGAGAACGAGATTGGTAAGAAGTCTCTGGT
AAGTCGGATCCTTAACGGACAGCGCACGTTAACGCTAGATCATATGCGAGCGCTGGCGAAGCGTTTCGGTTTGCCGGTGA
GCGCATTTGTGGGGAACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J0Q486

Antitoxin

Source ID Structure
AlphaFold DB A0A2J0Q1H0

References