Detailed information of TA system TA066085

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 485106..485620
• Replicon: chromosome
• Accession: NZ_CP017151
• Organism: Limosilactobacillus fermentum strain NCC2970

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: -
• Locus tag: LACFE_RS02445
• Protein ID: WP_069775721.1
• Coordinates: 485106..485369 (-)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: C0WV67
• Locus tag: LACFE_RS02450
• Protein ID: WP_003684069.1
• Coordinates: 485366..485620 (-)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LACFE_RS02430	480461..481501	-	1041	WP_015639354.1	Gfo/Idh/MocA family oxidoreductase	-
LACFE_RS02435	482368..483087	+	720	WP_069775720.1	hypothetical protein	-
LACFE_RS02440	483575..484696	+	1122	WP_023465987.1	PTS sugar transporter subunit IIC	-
LACFE_RS02445	485106..485369	-	264	WP_069775721.1	Txe/YoeB family addiction module toxin	Toxin
LACFE_RS02450	485366..485620	-	255	WP_003684069.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
LACFE_RS02455	485869..486156	+	288	WP_041807836.1	transposase	-
LACFE_RS02460	486180..487058	+	879	WP_069775722.1	IS3 family transposase	-
LACFE_RS09625	487856..488620	+	765	WP_080650602.1	zinc ribbon domain-containing protein	-
LACFE_RS02470	488745..489638	-	894	WP_069775723.1	IS3 family transposase	-
LACFE_RS02475	489662..489949	-	288	WP_003688751.1	transposase	-
LACFE_RS02480	490032..490568	-	537	Protein_486	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (26-86)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (3-55)

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 10303.00 Da
• Isoelectric Point: 10.3799

>T66085 WP_069775721.1 NZ_CP017151:c485369-485106 [Limosilactobacillus fermentum]
MSYAIKLKRSANKELKKIKGSYLEKSFLQIIEQLRKDTLAPNQGFEKLVPPIKGFYSRRINIQHRLVYKVDQDTQTVIIY
SAWSHYE

Nucleotide

• Length: 264 bp

>T66085 NZ_CP017151:c485369-485106 [Limosilactobacillus fermentum]
ATGAGTTACGCGATCAAACTCAAGCGGAGCGCTAATAAAGAACTTAAGAAAATTAAGGGGTCATATTTAGAAAAGAGCTT
CTTGCAAATAATTGAGCAGCTCCGTAAAGACACCTTGGCACCCAACCAAGGCTTTGAAAAATTGGTACCGCCAATTAAGG
GCTTTTACTCCCGGCGGATTAACATTCAGCACCGACTCGTCTATAAGGTTGATCAAGACACGCAAACTGTGATCATCTAT
TCTGCGTGGTCACATTACGAATAG

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9745.04 Da
• Isoelectric Point: 4.4237

>AT66085 WP_003684069.1 NZ_CP017151:c485620-485366 [Limosilactobacillus fermentum]
MDQIVTPTQARKDLFKIIKRINENNEPVVIKPTKADEKSVVVIGEDDWKAIQETLFLVNHGVDKQIKERENEPLDDFDDV
WGQL

Nucleotide

• Length: 255 bp

>AT66085 NZ_CP017151:c485620-485366 [Limosilactobacillus fermentum]
ATGGACCAAATTGTAACCCCAACGCAAGCACGGAAAGATTTATTCAAAATAATCAAAAGGATCAATGAAAATAATGAGCC
TGTTGTGATTAAGCCGACCAAGGCGGATGAAAAGAGCGTTGTCGTCATTGGTGAAGATGACTGGAAAGCAATCCAAGAAA
CGTTATTTTTGGTTAATCACGGTGTTGATAAGCAAATTAAAGAACGGGAAAACGAGCCGCTCGACGACTTTGATGATGTT
TGGGGTCAACTATGA

Structures

Antitoxin

• AlphaFold DB: A0A806T868