Detailed information of TA system TA066084

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 1697701..1698282
• Replicon: chromosome
• Accession: NZ_CP017150
• Organism: Brevibacterium aurantiacum strain SMQ-1335

Toxin (Protein)

• Gene name: graT
• Uniprot ID: A0A1D7W2M5
• Locus tag: BLSMQ_RS07755
• Protein ID: WP_069599926.1
• Coordinates: 1697701..1697982 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: BLSMQ_RS07760
• Protein ID: WP_069599927.1
• Coordinates: 1697992..1698282 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BLSMQ_RS07735	1692792..1693850	+	1059	WP_009883712.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
BLSMQ_RS07740	1693863..1695047	-	1185	WP_009883713.1	DNA recombination protein RmuC	-
BLSMQ_RS07745	1695121..1696206	+	1086	WP_069599924.1	redox-regulated ATPase YchF	-
BLSMQ_RS07750	1696199..1696564	+	366	WP_069599925.1	alkylphosphonate utilization protein	-
BLSMQ_RS07755	1697701..1697982	+	282	WP_069599926.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BLSMQ_RS07760	1697992..1698282	+	291	WP_069599927.1	HigA family addiction module antidote protein	Antitoxin
BLSMQ_RS07765	1698325..1699515	+	1191	WP_069599928.1	PD-(D/E)XK nuclease family protein	-
BLSMQ_RS07770	1699592..1700632	-	1041	WP_069601319.1	methionine synthase	-
BLSMQ_RS07775	1700650..1701642	-	993	WP_069599929.1	DUF1852 domain-containing protein	-
BLSMQ_RS07780	1701768..1702709	+	942	WP_029417202.1	LysR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (12-70)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10947.35 Da
• Isoelectric Point: 11.1409

>T66084 WP_069599926.1 NZ_CP017150:1697701-1697982 [Brevibacterium aurantiacum]
VIRSFADRDTERVWRREPARRLDPRIHKTANRKLHQLDAAVSLDSMRVPPGNRLESLRGDRAGQRSIRINDQWRICFRWT
GAGAEEVEIVDYH

Nucleotide

• Length: 282 bp

>T66084 NZ_CP017150:1697701-1697982 [Brevibacterium aurantiacum]
GTGATCAGATCGTTTGCCGACCGCGACACCGAGCGTGTGTGGCGTCGTGAACCGGCGAGGCGACTTGATCCTCGTATTCA
CAAGACTGCGAATCGGAAGTTGCATCAGCTGGATGCAGCGGTGTCGCTCGATTCCATGCGTGTTCCGCCCGGTAATCGTC
TGGAGTCACTCAGAGGTGATCGGGCTGGTCAGCGTAGCATTCGCATCAACGATCAGTGGCGGATCTGCTTTAGGTGGACC
GGTGCCGGTGCAGAAGAAGTCGAGATCGTGGACTATCACTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10834.46 Da
• Isoelectric Point: 5.1023

>AT66084 WP_069599927.1 NZ_CP017150:1697992-1698282 [Brevibacterium aurantiacum]
MSEKLYPPIHPGEVLMEDFIEGFGITQNKLAVSIGVPPRRINEIVHGKRAITADTALRLGRYFGVEPQFWLNLQSRYDLE
LAEERVSDQVAAITPL

Nucleotide

• Length: 291 bp

>AT66084 NZ_CP017150:1697992-1698282 [Brevibacterium aurantiacum]
ATGTCCGAGAAGCTTTACCCGCCGATCCATCCGGGGGAAGTCCTCATGGAGGATTTCATCGAGGGGTTCGGTATCACGCA
GAATAAGCTCGCTGTGTCGATCGGTGTGCCACCTCGGCGTATCAATGAAATAGTGCACGGAAAGCGTGCCATCACTGCCG
ACACAGCTTTGCGTTTGGGGCGGTATTTCGGTGTGGAGCCGCAATTCTGGCTTAACCTGCAGAGTCGTTATGATCTGGAG
CTTGCAGAGGAACGCGTGTCCGACCAGGTCGCGGCAATCACGCCGCTTTAG

Structures

Toxin

• AlphaFold DB: A0A1D7W2M5