Detailed information of TA system TA066083

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5253822..5254417
• Replicon: chromosome
• Accession: NZ_CP017149
• Organism: Pseudomonas aeruginosa strain ATCC 15692

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: BGV84_RS29675
• Protein ID: WP_003113526.1
• Coordinates: 5254139..5254417 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: BGV84_RS24205
• Protein ID: WP_003113527.1
• Coordinates: 5253822..5254127 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BGV84_RS29645	5248935..5249225	-	291	WP_033992920.1	DUF5447 family protein	-
BGV84_RS24200	5249437..5249709	-	273	WP_003115921.1	hypothetical protein	-
BGV84_RS29660	5250239..5251336	+	1098	WP_169917322.1	protein phosphatase 2C domain-containing protein	-
BGV84_RS29665	5251339..5252388	+	1050	WP_003113529.1	serine/threonine protein kinase	-
BGV84_RS29670	5252385..5253458	+	1074	WP_003113528.1	serine/threonine protein kinase	-
BGV84_RS24205	5253822..5254127	-	306	WP_003113527.1	HigA family addiction module antidote protein	Antitoxin
BGV84_RS29675	5254139..5254417	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BGV84_RS24210	5254746..5256974	+	2229	WP_003113525.1	TonB-dependent receptor	-
BGV84_RS24215	5257044..5257691	-	648	WP_003095021.1	carbonate dehydratase	-
BGV84_RS24220	5257753..5258991	-	1239	WP_003113524.1	dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T66083 WP_003113526.1 NZ_CP017149:c5254417-5254139 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T66083 NZ_CP017149:c5254417-5254139 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11247.81 Da
• Isoelectric Point: 5.0747

>AT66083 WP_003113527.1 NZ_CP017149:c5254127-5253822 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT66083 NZ_CP017149:c5254127-5253822 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTTGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: V6ALY3