Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mqsRA (relBE)/MqsR-MqsA
Location 3108397..3109090 Replicon chromosome
Accession NZ_CP017100
Organism Escherichia coli strain K-12 NEB 5-alpha

Toxin (Protein)


Gene name mqsR Uniprot ID S1EZG2
Locus tag NEB5A_RS15445 Protein ID WP_000415584.1
Coordinates 3108794..3109090 (-) Length 99 a.a.

Antitoxin (Protein)


Gene name mqsA Uniprot ID S1EBV2
Locus tag NEB5A_RS15440 Protein ID WP_000650107.1
Coordinates 3108397..3108792 (-) Length 132 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NEB5A_RS15430 3104261..3106519 - 2259 WP_001281881.1 DNA topoisomerase IV subunit A -
NEB5A_RS15435 3106657..3108264 - 1608 WP_001295629.1 ABC transporter substrate-binding protein -
NEB5A_RS15440 3108397..3108792 - 396 WP_000650107.1 type II toxin-antitoxin system antitoxin MqsA Antitoxin
NEB5A_RS15445 3108794..3109090 - 297 WP_000415584.1 type II toxin-antitoxin system toxin MqsR Toxin
NEB5A_RS15450 3109295..3109777 - 483 WP_000183505.1 transcriptional regulator -
NEB5A_RS15455 3109830..3110222 - 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
NEB5A_RS15460 3110374..3111033 + 660 WP_001221495.1 two-component system response regulator QseB -
NEB5A_RS15465 3111030..3112379 + 1350 WP_000673402.1 two-component system sensor histidine kinase QseC -
NEB5A_RS15470 3112425..3112757 - 333 WP_000917684.1 DUF2645 family protein -
NEB5A_RS15475 3113076..3113657 + 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
NEB5A_RS15480 3113688..3114002 + 315 WP_000958598.1 antibiotic biosynthesis monooxygenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin MqsR

(5-97)

Antitoxin

Zinc finger, MqsA-type

(3-47)


Sequences

Toxin        


Download         Length: 99 a.a.        Molecular weight: 11231.96 Da        Isoelectric Point: 8.9070

>T65993 WP_000415584.1 NZ_CP017100:c3109090-3108794 [Escherichia coli]
MEKRTPHTRLSQVKKLVNAGQVRTTRSALLNADELGLDFDGMCNVIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQV
YLKITVIHDVLIVSFKEK

Download         Length: 297 bp

>T65993 NZ_CP017100:c3109090-3108794 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin


Download         Length: 132 a.a.        Molecular weight: 14703.10 Da        Isoelectric Point: 9.2136

>AT65993 WP_000650107.1 NZ_CP017100:c3108792-3108397 [Escherichia coli]
MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESIMNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKK
LSLTQKEASEIFGGGVNAFSRYEKGNAQPHPSTIKLLRVLDKHPELLNEIR

Download         Length: 396 bp

>AT65993 NZ_CP017100:c3108792-3108397 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 3HI2

Antitoxin

Source ID Structure
PDB 3HI2
PDB 3GA8
PDB 3O9X
PDB 2KZ8
PDB 3FMY
PDB 3GN5
AlphaFold DB A0A7U9LU65

References