65988

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2035518..2036020 Replicon chromosome
Accession NZ_CP017100
Organism Escherichia coli strain K-12 NEB 5-alpha

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag NEB5A_RS10345 Protein ID WP_000767829.1
Coordinates 2035518..2035772 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag NEB5A_RS10350 Protein ID WP_001259255.1
Coordinates 2035769..2036020 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NEB5A_RS10320 2030533..2030760 - 228 WP_046613325.1 sulfurtransferase TusA family protein -
NEB5A_RS10325 2030774..2031832 - 1059 WP_000492339.1 transport protein YeeE -
NEB5A_RS10330 2032011..2033369 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
NEB5A_RS25220 2033359..2033421 - 63 WP_010723108.1 membrane protein YoeI -
NEB5A_RS10335 2033636..2034565 - 930 WP_000803351.1 LysR family transcriptional regulator -
NEB5A_RS10340 2034611..2035435 - 825 WP_000754737.1 SDR family oxidoreductase -
NEB5A_RS10345 2035518..2035772 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
NEB5A_RS10350 2035769..2036020 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
NEB5A_RS23580 2036303..2036353 + 51 WP_001364200.1 his operon leader peptide -
NEB5A_RS10355 2036499..2037398 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
NEB5A_RS10360 2037404..2038708 + 1305 WP_000009594.1 histidinol dehydrogenase -
NEB5A_RS10365 2038705..2039775 + 1071 WP_000108941.1 histidinol-phosphate transaminase -
NEB5A_RS10370 2039775..2040842 + 1068 WP_000080105.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T65988 WP_000767829.1 NZ_CP017100:c2035772-2035518 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T65988 NZ_CP017100:c2035772-2035518 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT65988 WP_001259255.1 NZ_CP017100:c2036020-2035769 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT65988 NZ_CP017100:c2036020-2035769 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LNR0

References