Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2035518..2036020 Replicon chromosome
Accession NZ_CP017100
Organism Escherichia coli strain K-12 NEB 5-alpha

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag NEB5A_RS10345 Protein ID WP_000767829.1
Coordinates 2035518..2035772 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag NEB5A_RS10350 Protein ID WP_001259255.1
Coordinates 2035769..2036020 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NEB5A_RS10320 2030533..2030760 - 228 WP_046613325.1 sulfurtransferase TusA family protein -
NEB5A_RS10325 2030774..2031832 - 1059 WP_000492339.1 transport protein YeeE -
NEB5A_RS10330 2032011..2033369 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
NEB5A_RS25220 2033359..2033421 - 63 WP_010723108.1 membrane protein YoeI -
NEB5A_RS10335 2033636..2034565 - 930 WP_000803351.1 LysR family transcriptional regulator -
NEB5A_RS10340 2034611..2035435 - 825 WP_000754737.1 SDR family oxidoreductase -
NEB5A_RS10345 2035518..2035772 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
NEB5A_RS10350 2035769..2036020 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
NEB5A_RS23580 2036303..2036353 + 51 WP_001364200.1 his operon leader peptide -
NEB5A_RS10355 2036499..2037398 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
NEB5A_RS10360 2037404..2038708 + 1305 WP_000009594.1 histidinol dehydrogenase -
NEB5A_RS10365 2038705..2039775 + 1071 WP_000108941.1 histidinol-phosphate transaminase -
NEB5A_RS10370 2039775..2040842 + 1068 WP_000080105.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T65988 WP_000767829.1 NZ_CP017100:c2035772-2035518 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T65988 NZ_CP017100:c2035772-2035518 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT65988 WP_001259255.1 NZ_CP017100:c2036020-2035769 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT65988 NZ_CP017100:c2036020-2035769 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References