Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 253343..254037 Replicon chromosome
Accession NZ_CP017100
Organism Escherichia coli strain K-12 NEB 5-alpha

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag NEB5A_RS01200 Protein ID WP_001263489.1
Coordinates 253639..254037 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag NEB5A_RS01195 Protein ID WP_000554758.1
Coordinates 253343..253636 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NEB5A_RS01175 248975..249472 + 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
NEB5A_RS01180 249696..251408 - 1713 Protein_227 flagellar biosynthesis protein FlhA -
NEB5A_RS01185 251380..252165 + 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
NEB5A_RS01190 252236..253291 + 1056 WP_001226164.1 DNA polymerase IV -
NEB5A_RS01195 253343..253636 + 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
NEB5A_RS01200 253639..254037 + 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
NEB5A_RS01205 254047..254499 + 453 WP_001059892.1 GNAT family N-acetyltransferase -
NEB5A_RS01210 254817..255023 + 207 Protein_233 RtcB family protein -
NEB5A_RS01215 255019..255540 + 522 Protein_234 peptide chain release factor H -
NEB5A_RS01220 255597..257054 - 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
NEB5A_RS01225 257315..257773 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -
- 258369..258449 + 81 NuclAT_12 - -
- 258369..258449 + 81 NuclAT_12 - -
- 258369..258449 + 81 NuclAT_12 - -
- 258369..258449 + 81 NuclAT_12 - -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T65963 WP_001263489.1 NZ_CP017100:253639-254037 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T65963 NZ_CP017100:253639-254037 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT65963 WP_000554758.1 NZ_CP017100:253343-253636 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT65963 NZ_CP017100:253343-253636 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References