Detailed information of TA system TA065903

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1920653..1921310
• Replicon: chromosome
• Accession: NZ_CP017087
• Organism: Enterobacter sp. HK169

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: EnteroDNA1_RS09010
• Protein ID: WP_064673553.1
• Coordinates: 1920653..1920952 (+)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A808ICP3
• Locus tag: EnteroDNA1_RS09015
• Protein ID: WP_033146339.1
• Coordinates: 1920963..1921310 (+)
• Length: 116 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EnteroDNA1_RS09000	1917022..1919220	+	2199	WP_069303204.1	type I secretion system permease/ATPase	-
EnteroDNA1_RS09005	1919231..1920400	+	1170	WP_086374946.1	HlyD family efflux transporter periplasmic adaptor subunit	-
EnteroDNA1_RS09010	1920653..1920952	+	300	WP_064673553.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EnteroDNA1_RS09015	1920963..1921310	+	348	WP_033146339.1	HigA family addiction module antidote protein	Antitoxin
EnteroDNA1_RS09020	1921355..1921780	+	426	WP_064673552.1	nucleoside triphosphatase NudI	-
EnteroDNA1_RS09025	1921752..1922483	-	732	WP_069303205.1	helix-turn-helix transcriptional regulator	-
EnteroDNA1_RS09030	1922581..1923000	+	420	WP_039263434.1	nuclear transport factor 2 family protein	-
EnteroDNA1_RS09035	1923003..1923521	-	519	WP_069303206.1	hypothetical protein	-
EnteroDNA1_RS09040	1923606..1924787	-	1182	WP_069303207.1	PLP-dependent aminotransferase family protein	-
EnteroDNA1_RS09045	1924807..1925694	-	888	WP_069303208.1	LysR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-93)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (8-86)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11721.35 Da
• Isoelectric Point: 10.0346

>T65903 WP_064673553.1 NZ_CP017087:1920653-1920952 [Enterobacter sp. HK169]
MISYFRDQWLEDFFLYGRSSNVIPANLETTLARKLDIIRAATSHRDLRSPPGNMYEALNPPLKGYSSIRVNRQYRLVFRW
TEGKAEDLYLSPHKYTQHK

Nucleotide

• Length: 300 bp

>T65903 NZ_CP017087:1920653-1920952 [Enterobacter sp. HK169]
ATGATTAGTTATTTTCGGGATCAATGGCTTGAGGATTTTTTTCTTTACGGCAGATCGAGCAATGTTATTCCTGCAAATCT
GGAAACAACGCTTGCGAGAAAGCTCGATATCATCAGGGCAGCAACCTCGCACAGGGATTTGCGATCGCCACCGGGCAATA
TGTATGAAGCATTGAATCCTCCGCTGAAGGGATATTCCTCTATCCGGGTAAACAGGCAATACAGGCTCGTATTTCGCTGG
ACAGAGGGTAAAGCAGAAGATCTCTACCTCTCTCCACACAAGTACACGCAACACAAGTGA

Antitoxin

Protein

• Length: 116 a.a.
• Molecular weight: 13066.02 Da
• Isoelectric Point: 7.0134

>AT65903 WP_033146339.1 NZ_CP017087:1920963-1921310 [Enterobacter sp. HK169]
MTLQQALRKPTTPGDVLQYEYLEPLNLKISDLAEMLNVHRNTISALVNNNRKLTADMAIKLAKAFDTTIEFWLNLQLNVD
IWEAQSNPRTQEELSRIKTVADVMAKRKSGQPDVA

Nucleotide

• Length: 348 bp

>AT65903 NZ_CP017087:1920963-1921310 [Enterobacter sp. HK169]
ATGACACTTCAACAGGCACTCCGCAAACCCACCACGCCGGGCGACGTGTTGCAGTATGAGTATCTTGAACCGCTCAATCT
GAAAATCAGCGATCTGGCGGAGATGCTCAATGTTCACCGCAACACCATCAGCGCGCTGGTCAATAACAATCGCAAGCTTA
CTGCCGATATGGCGATCAAACTGGCAAAAGCCTTTGATACCACGATTGAATTTTGGCTTAACTTACAGCTGAACGTCGAT
ATCTGGGAAGCGCAATCCAACCCCAGGACGCAGGAGGAGCTAAGCCGTATAAAGACCGTTGCGGACGTCATGGCTAAGCG
AAAATCTGGCCAGCCGGATGTAGCCTGA

Structures

Antitoxin

• AlphaFold DB: A0A808ICP3