Detailed information of TA system TA065901

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 1294759..1295419
• Replicon: chromosome
• Accession: NZ_CP017087
• Organism: Enterobacter sp. HK169

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: EnteroDNA1_RS06260
• Protein ID: WP_069303044.1
• Coordinates: 1295066..1295419 (-)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A1Z3MX79
• Locus tag: EnteroDNA1_RS06255
• Protein ID: WP_029741460.1
• Coordinates: 1294759..1295061 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EnteroDNA1_RS06225	1289866..1292055	+	2190	WP_045356712.1	TonB-dependent siderophore receptor	-
EnteroDNA1_RS06235	1292350..1292682	-	333	WP_064672767.1	type II toxin-antitoxin system PemK/MazF family toxin	-
EnteroDNA1_RS06240	1292683..1292940	-	258	WP_033145928.1	antitoxin	-
EnteroDNA1_RS06245	1293232..1293621	-	390	WP_059445031.1	RidA family protein	-
EnteroDNA1_RS06250	1293763..1294683	+	921	WP_069303043.1	LysR family transcriptional regulator	-
EnteroDNA1_RS06255	1294759..1295061	-	303	WP_029741460.1	XRE family transcriptional regulator	Antitoxin
EnteroDNA1_RS06260	1295066..1295419	-	354	WP_069303044.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EnteroDNA1_RS06265	1295597..1296490	-	894	WP_069303045.1	LysR family transcriptional regulator	-
EnteroDNA1_RS06270	1296661..1297269	+	609	WP_083239546.1	glutathione S-transferase family protein	-
EnteroDNA1_RS06275	1297313..1298263	-	951	WP_023336219.1	HTH-type transcriptional regulator Cbl	-
EnteroDNA1_RS06280	1298360..1299277	-	918	WP_033145933.1	nitrogen assimilation transcriptional regulator NAC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1292683..1304288	11605

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 13504.29 Da
• Isoelectric Point: 7.1680

>T65901 WP_069303044.1 NZ_CP017087:c1295419-1295066 [Enterobacter sp. HK169]
VWAINTTDRFDRWFTSLDDTERASVLAALLVLREKGPGLSRPYADTLKGSRHSNMKELRIQSKGDPLRAFFAFDPNRTGI
VLCAGNKVGNERRFYDEMLLVADQEFTHWLDTLKERE

Nucleotide

• Length: 354 bp

>T65901 NZ_CP017087:c1295419-1295066 [Enterobacter sp. HK169]
GTGTGGGCAATTAACACGACGGACAGGTTTGATCGCTGGTTCACTTCGCTTGACGATACCGAACGCGCCAGCGTACTTGC
AGCACTGCTTGTTTTGCGAGAGAAGGGGCCGGGTTTATCGAGACCCTACGCTGATACGCTTAAAGGCTCTCGTCACAGCA
ACATGAAAGAGCTACGTATTCAGAGTAAGGGCGATCCCTTGCGGGCATTTTTCGCCTTTGATCCAAACCGCACGGGTATC
GTGCTTTGTGCGGGCAATAAGGTCGGCAATGAAAGGCGATTCTACGATGAGATGCTTCTCGTTGCTGACCAGGAATTCAC
TCACTGGCTGGATACATTAAAAGAGAGGGAATAG

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11050.83 Da
• Isoelectric Point: 5.7316

>AT65901 WP_029741460.1 NZ_CP017087:c1295061-1294759 [Enterobacter sp. HK169]
MGRTLEQLIADEKPEVVASAQAIATDILLNIHLAELREKVQKTQVEMAQALGIRQPTVAVMEKPGRDLKLSSLKRYVEAA
GGKLRLDVELPDGSHYEFVL

Nucleotide

• Length: 303 bp

>AT65901 NZ_CP017087:c1295061-1294759 [Enterobacter sp. HK169]
ATGGGAAGAACCCTTGAGCAGCTTATAGCGGATGAAAAGCCGGAGGTTGTCGCCAGCGCTCAGGCGATAGCAACGGATAT
TTTGCTCAACATTCATCTTGCTGAGTTACGCGAGAAAGTACAAAAAACGCAGGTAGAAATGGCGCAGGCGTTGGGTATCA
GGCAACCTACCGTTGCGGTAATGGAAAAGCCCGGGCGCGATTTGAAACTCTCTTCTCTCAAGCGCTACGTTGAGGCGGCG
GGCGGAAAGCTGCGGCTTGATGTGGAGCTGCCGGACGGCTCGCACTATGAGTTTGTGCTGTAG

Structures

Antitoxin

• AlphaFold DB: A0A1Z3MX79