Detailed information of TA system TA065888

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 35779..36032
• Replicon: plasmid pT18
• Accession: NZ_CP017086
• Organism: Proteus mirabilis strain T18

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: BG029_RS19810
• Protein ID: WP_001312851.1
• Coordinates: 35883..36032 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	35779..35838 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BG029_RS19765	31439..31504	-	66	Protein_37	helix-turn-helix domain-containing protein	-
BG029_RS19770	31557..32261	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
BG029_RS19780	32506..33252	+	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
BG029_RS19785	33307..33867	+	561	WP_000139328.1	fertility inhibition protein FinO	-
BG029_RS19790	33999..34199	+	201	WP_015059022.1	hypothetical protein	-
BG029_RS19795	34585..35184	+	600	WP_032083981.1	hypothetical protein	-
-	35779..35838	-	60	NuclAT_0	-	Antitoxin
-	35779..35838	-	60	NuclAT_0	-	Antitoxin
-	35779..35838	-	60	NuclAT_0	-	Antitoxin
-	35779..35838	-	60	NuclAT_0	-	Antitoxin
BG029_RS19810	35883..36032	+	150	WP_001312851.1	type I toxin-antitoxin system Hok family toxin	Toxin
BG029_RS19815	36316..36564	+	249	WP_000083837.1	replication regulatory protein RepA	-
BG029_RS19825	36809..36883	+	75	WP_001375168.1	RepA leader peptide Tap	-
BG029_RS19830	36876..37733	+	858	WP_032495102.1	incFII family plasmid replication initiator RepA	-
BG029_RS19840	38672..39325	+	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
BG029_RS20195	39418..39675	+	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
BG029_RS19845	39608..40009	+	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
BG029_RS19855	40258..40673	+	416	Protein_50	IS1 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaKPC-2 / blaCTX-M-65 / rmtB / blaTEM-1B / fosA3	-	1..59035	59035

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T65888 WP_001312851.1 NZ_CP017086:35883-36032 [Proteus mirabilis]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T65888 NZ_CP017086:35883-36032 [Proteus mirabilis]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGTGCCACGGTATTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 60 bp

>AT65888 NZ_CP017086:c35838-35779 [Proteus mirabilis]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin