Detailed information of TA system TA065880

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 144729..145386
• Replicon: plasmid pT212
• Accession: NZ_CP017084
• Organism: Proteus mirabilis strain T21

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U3PDC3
• Locus tag: BG257_RS20340
• Protein ID: WP_000270043.1
• Coordinates: 145036..145386 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: BG257_RS20335
• Protein ID: WP_000124640.1
• Coordinates: 144729..145031 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BG257_RS20285	140354..140782	+	429	WP_000591074.1	hypothetical protein	-
BG257_RS20290	140839..141198	+	360	WP_000422768.1	hypothetical protein	-
BG257_RS20295	141198..141644	+	447	WP_000919345.1	Fe3+-siderophore ABC transporter permease	-
BG257_RS20300	141641..142159	+	519	WP_000210756.1	nitrite reductase	-
BG257_RS20305	142159..142389	+	231	WP_096043120.1	hypothetical protein	-
BG257_RS20310	142376..143233	+	858	WP_001167032.1	hypothetical protein	-
BG257_RS20320	143464..143991	+	528	WP_001236377.1	thermonuclease family protein	-
BG257_RS20325	144049..144321	+	273	WP_001043047.1	HU family DNA-binding protein	-
BG257_RS20330	144409..144702	+	294	WP_001239997.1	chromosome segregation protein ParM	-
BG257_RS20335	144729..145031	-	303	WP_000124640.1	XRE family transcriptional regulator	Antitoxin
BG257_RS20340	145036..145386	-	351	WP_000270043.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BG257_RS20345	145549..146097	+	549	WP_001061574.1	transcriptional regulator	-
BG257_RS20355	146438..146632	+	195	WP_000343597.1	hypothetical protein	-
BG257_RS20360	146643..147014	+	372	WP_000516916.1	hypothetical protein	-
BG257_RS20365	147007..147477	+	471	WP_001281821.1	hypothetical protein	-
BG257_RS20370	147492..147827	-	336	WP_000683476.1	hypothetical protein	-
BG257_RS20375	147924..148412	+	489	WP_001273096.1	DUF1643 domain-containing protein	-
BG257_RS20380	148415..148912	+	498	WP_000062185.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-14 / sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1B / aac(3)-IId / dfrA12 / aadA2 / aph(3')-Ia / rmtB / tet(G)	-	1..171489	171489

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13328.21 Da
• Isoelectric Point: 5.2421

>T65880 WP_000270043.1 NZ_CP017084:c145386-145036 [Proteus mirabilis]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Nucleotide

• Length: 351 bp

>T65880 NZ_CP017084:c145386-145036 [Proteus mirabilis]
ATGTGGGTCATCGAGACAACCGACACCTTTGATGAGTGGTTCGATGCTCTGGATGATACCGATAGAGCAAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGCCCCATGCTGTCGAGGCCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTTCGGGTCCAAAGCAAAGGAGATCCTATCAGAGCGTTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGTGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11006.73 Da
• Isoelectric Point: 7.2036

>AT65880 WP_000124640.1 NZ_CP017084:c145031-144729 [Proteus mirabilis]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Nucleotide

• Length: 303 bp

>AT65880 NZ_CP017084:c145031-144729 [Proteus mirabilis]
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA

Structures

Toxin

• AlphaFold DB: A0A7G8AFB9