65852

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-DinJ
Location 466040..466525 Replicon chromosome
Accession NZ_CP017063
Organism Lacticaseibacillus rhamnosus strain LR5

Toxin (Protein)


Gene name yafQ Uniprot ID C2K0Y1
Locus tag BGK71_RS02310 Protein ID WP_005691636.1
Coordinates 466040..466225 (-) Length 62 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID A0A249DC59
Locus tag BGK71_RS02315 Protein ID WP_047676897.1
Coordinates 466253..466525 (-) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BGK71_RS02285 461047..461736 + 690 WP_047676891.1 rhomboid family intramembrane serine protease -
BGK71_RS02290 462228..462836 - 609 WP_047676893.1 hypothetical protein -
BGK71_RS02300 463283..464158 + 876 WP_024305787.1 XRE family transcriptional regulator -
BGK71_RS02305 464401..465816 - 1416 WP_064535168.1 DNA polymerase III subunit epsilon -
BGK71_RS02310 466040..466225 - 186 WP_005691636.1 type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family Toxin
BGK71_RS02315 466253..466525 - 273 WP_047676897.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
BGK71_RS02320 467087..467335 + 249 WP_005691639.1 hypothetical protein -
BGK71_RS02325 467578..467871 + 294 WP_005684269.1 metalloregulator ArsR/SmtB family transcription factor -
BGK71_RS02330 467953..469116 + 1164 WP_005691640.1 MFS transporter -
BGK71_RS02335 469270..469845 + 576 WP_047676900.1 DNA-3-methyladenine glycosylase I -
BGK71_RS02340 469967..470185 + 219 WP_047676903.1 hypothetical protein -
BGK71_RS02345 470314..471195 - 882 WP_005684263.1 class II fructose-1,6-bisphosphate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(8-57)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-84)


Sequences

Toxin        


Download         Length: 62 a.a.        Molecular weight: 6781.86 Da        Isoelectric Point: 7.4698

>T65852 WP_005691636.1 NZ_CP017063:c466225-466040 [Lacticaseibacillus rhamnosus]
MQRQGHVKLASLHDHALKGAHSGERALRVAPDWLLVYKVDAEALILMLLATGTHRDTLNIE

Download         Length: 186 bp

>T65852 NZ_CP017063:c466225-466040 [Lacticaseibacillus rhamnosus]
TTGCAGCGTCAAGGTCATGTAAAATTAGCTTCTCTACATGACCATGCTTTAAAGGGTGCTCACAGCGGCGAACGAGCATT
GCGTGTTGCTCCTGATTGGCTTCTCGTTTATAAAGTCGATGCTGAAGCGTTAATTTTAATGCTTCTTGCAACAGGCACAC
ACCGCGATACATTGAACATTGAGTAA

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 9979.58 Da        Isoelectric Point: 9.5939

>AT65852 WP_047676897.1 NZ_CP017063:c466525-466253 [Lacticaseibacillus rhamnosus]
MTKKSRISVRIDTKTKERALHVLNSMGLDISSAINMYLKRIGDTGALPFTPAMSFVDQLQVAEADVKAGRIKSFKTVDAL
MKDLYSDVDD

Download         Length: 273 bp

>AT65852 NZ_CP017063:c466525-466253 [Lacticaseibacillus rhamnosus]
ATGACCAAAAAATCCCGCATCAGCGTTAGGATTGATACCAAAACTAAAGAACGAGCGCTCCATGTTCTTAATAGCATGGG
ATTAGATATTTCCTCGGCTATTAACATGTACCTGAAACGGATTGGTGACACCGGTGCATTGCCATTTACACCTGCAATGT
CATTTGTTGATCAGCTTCAAGTTGCAGAAGCTGATGTTAAAGCGGGGCGAATAAAAAGCTTCAAGACTGTCGATGCTTTG
ATGAAGGATTTATACAGTGATGTTGACGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7X2J6C9

Antitoxin

Source ID Structure
AlphaFold DB A0A249DC59

References