Detailed information of TA system TA065848

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 41627..42284
• Replicon: plasmid unnamed1
• Accession: NZ_CP017058
• Organism: Citrobacter freundii strain SL151

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U3PDC3
• Locus tag: BFQ28_RS24390
• Protein ID: WP_000270043.1
• Coordinates: 41627..41977 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: BFQ28_RS24395
• Protein ID: WP_000124640.1
• Coordinates: 41982..42284 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BFQ28_RS24345	37131..37835	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
BFQ28_RS24350	38101..38598	-	498	WP_000062185.1	hypothetical protein	-
BFQ28_RS24355	38601..39089	-	489	WP_001273096.1	DUF1643 domain-containing protein	-
BFQ28_RS24360	39186..39521	+	336	WP_000683476.1	hypothetical protein	-
BFQ28_RS24365	39536..40006	-	471	WP_001281821.1	hypothetical protein	-
BFQ28_RS28215	39999..40370	-	372	WP_000516916.1	hypothetical protein	-
BFQ28_RS28220	40381..40575	-	195	WP_000343597.1	hypothetical protein	-
BFQ28_RS24385	40916..41464	-	549	WP_001061574.1	transcriptional regulator	-
BFQ28_RS24390	41627..41977	+	351	WP_000270043.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BFQ28_RS24395	41982..42284	+	303	WP_000124640.1	XRE family transcriptional regulator	Antitoxin
BFQ28_RS24400	42311..42604	-	294	WP_001239997.1	chromosome segregation protein ParM	-
BFQ28_RS24405	42692..42964	-	273	WP_001043047.1	HU family DNA-binding protein	-
BFQ28_RS24410	43022..43549	-	528	WP_001236377.1	thermonuclease family protein	-
BFQ28_RS24415	43566..43805	-	240	Protein_56	hypothetical protein	-
BFQ28_RS24420	43981..45189	+	1209	WP_069219771.1	site-specific integrase	-
BFQ28_RS24425	45228..45587	+	360	WP_069219772.1	type II toxin-antitoxin system RelE/ParE family toxin	-
BFQ28_RS24430	45591..45911	+	321	WP_069219773.1	helix-turn-helix domain-containing protein	-
BFQ28_RS24435	45946..46926	+	981	WP_069219774.1	hypothetical protein	-
BFQ28_RS24440	46895..47143	+	249	WP_069219775.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	catA1 / aac(3)-IIa / blaTEM-1B / blaCTX-M-15 / qnrS1 / sul2 / aph(6)-Id / aph(3'')-Ib	-	1..229406	229406

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13328.21 Da
• Isoelectric Point: 5.2421

>T65848 WP_000270043.1 NZ_CP017058:41627-41977 [Citrobacter freundii]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Nucleotide

• Length: 351 bp

>T65848 NZ_CP017058:41627-41977 [Citrobacter freundii]
ATGTGGGTCATCGAGACAACCGACACCTTTGATGAGTGGTTCGATGCTCTGGATGATACCGATAGAGCAAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGCCCCATGCTGTCGAGGCCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTTCGGGTCCAAAGCAAAGGAGATCCTATCAGAGCGTTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGTGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11006.73 Da
• Isoelectric Point: 7.2036

>AT65848 WP_000124640.1 NZ_CP017058:41982-42284 [Citrobacter freundii]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Nucleotide

• Length: 303 bp

>AT65848 NZ_CP017058:41982-42284 [Citrobacter freundii]
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA

Structures

Toxin

• AlphaFold DB: A0A7G8AFB9