Detailed information of TA system TA065807

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 3896154..3896749
• Replicon: chromosome
• Accession: NZ_CP017014
• Organism: Rhodococcus sp. WMMA185

Toxin (Protein)

• Gene name: graT
• Uniprot ID: -
• Locus tag: BFN03_RS20290
• Protein ID: WP_084385673.1
• Coordinates: 3896504..3896749 (-)
• Length: 82 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A1D8TC56
• Locus tag: BFN03_RS17570
• Protein ID: WP_070381039.1
• Coordinates: 3896154..3896465 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BFN03_RS17540	3891417..3891992	+	576	WP_070380082.1	TetR family transcriptional regulator	-
BFN03_RS17545	3892072..3892581	-	510	WP_070380083.1	hypothetical protein	-
BFN03_RS17550	3892592..3893170	-	579	WP_070381038.1	hypothetical protein	-
BFN03_RS17555	3893302..3893952	-	651	WP_070380084.1	enoyl-CoA hydratase/isomerase family protein	-
BFN03_RS17560	3893949..3894608	-	660	WP_070380085.1	TetR/AcrR family transcriptional regulator; helix-turn-helix transcriptional regulator	-
BFN03_RS17565	3894824..3896020	+	1197	WP_070380086.1	RtcB family protein	-
BFN03_RS17570	3896154..3896465	-	312	WP_070381039.1	HigA family addiction module antidote protein	Antitoxin
BFN03_RS20290	3896504..3896749	-	246	WP_084385673.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BFN03_RS20500	3896695..3896988	+	294	WP_157109645.1	RtcB family protein	-
BFN03_RS17580	3897016..3897567	+	552	WP_070380088.1	acetyltransferase	-
BFN03_RS17585	3897773..3898780	-	1008	WP_070380089.1	beta-propeller fold lactonase family protein	-
BFN03_RS17590	3899188..3901044	+	1857	WP_070380090.1	long-chain fatty acid--CoA ligase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (6-81)

Antitoxin

• Cro/C1-type helix-turn-helix domain (21-71)

Sequences

Toxin

Protein

• Length: 82 a.a.
• Molecular weight: 9430.69 Da
• Isoelectric Point: 9.0875

>T65807 WP_084385673.1 NZ_CP017014:c3896749-3896504 [Rhodococcus sp. WMMA185]
VWGRRHSRQLGPEVQRAAYRKLLILDAADDINDLRIPPGNRLEKLVGDRKGQHSIRIDDQWRICFVWTASGPRDVEIADY
H

Nucleotide

• Length: 246 bp

>T65807 NZ_CP017014:c3896749-3896504 [Rhodococcus sp. WMMA185]
GTGTGGGGCCGTCGCCACAGCCGCCAACTCGGCCCAGAGGTTCAGCGAGCGGCATACCGGAAACTGCTGATCCTCGACGC
CGCGGACGACATCAACGACCTTCGCATTCCGCCAGGTAACCGGCTGGAAAAGCTGGTAGGAGACCGTAAGGGGCAGCACA
GCATCCGAATCGACGATCAATGGCGGATCTGTTTCGTCTGGACCGCGAGCGGCCCCCGCGATGTGGAAATCGCCGACTAC
CACTGA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11924.63 Da
• Isoelectric Point: 6.7271

>AT65807 WP_070381039.1 NZ_CP017014:c3896465-3896154 [Rhodococcus sp. WMMA185]
MNAQNEPLFDRVTPGEILNEEYIKAFGITQHHLAVAIGVPPRRINEIVHGKRRISPDTALRLARYFSTSERFWMNLQDRY
DIEVERERIAPQLDAITPLVLNA

Nucleotide

• Length: 312 bp

>AT65807 NZ_CP017014:c3896465-3896154 [Rhodococcus sp. WMMA185]
ATGAACGCACAGAACGAACCACTTTTCGACCGGGTCACCCCAGGGGAGATCCTCAACGAGGAGTACATCAAGGCATTCGG
GATCACCCAGCACCACCTAGCTGTCGCGATCGGGGTTCCGCCGCGCCGGATCAACGAGATCGTGCACGGCAAGCGCCGTA
TCTCCCCGGACACTGCCCTCCGGCTGGCCCGGTACTTCTCGACCAGCGAACGGTTCTGGATGAACCTGCAGGACCGCTAC
GACATCGAAGTCGAGCGCGAGCGTATCGCGCCCCAATTGGACGCCATCACCCCGCTGGTGCTGAACGCGTAG

Structures

Antitoxin

• AlphaFold DB: A0A1D8TC56