Detailed information of TA system TA065775

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 2344646..2345225
• Replicon: chromosome
• Accession: NZ_CP016987
• Organism: Vibrio cholerae strain FORC_055 isolate MFDS

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: FORC55_RS11025
• Protein ID: WP_050553755.1
• Coordinates: 2344646..2344930 (+)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FORC55_RS11030
• Protein ID: WP_032475673.1
• Coordinates: 2344956..2345225 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FORC55_RS19835	2341163..2341351	-	189	Protein_2055	IS66 family transposase	-
FORC55_RS11010	2341550..2343094	+	1545	WP_032475666.1	methyl-accepting chemotaxis protein	-
FORC55_RS11015	2343127..2344251	-	1125	WP_001963943.1	IS630 family transposase	-
FORC55_RS11020	2344325..2344564	+	240	WP_001963942.1	MarR family transcriptional regulator	-
FORC55_RS11025	2344646..2344930	+	285	WP_050553755.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FORC55_RS11030	2344956..2345225	+	270	WP_032475673.1	XRE family transcriptional regulator	Antitoxin
FORC55_RS11035	2345723..2349292	-	3570	WP_001963938.1	conjugal transfer protein TraG N-terminal domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	2279843..2423108	143265

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (5-83)

Antitoxin

• Cro/C1-type helix-turn-helix domain (21-70)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10765.30 Da
• Isoelectric Point: 7.1354

>T65775 WP_050553755.1 NZ_CP016987:2344646-2344930 [Vibrio cholerae]
INVRACIQVLLERGPQLSRPYADTVEDSKHSNMKELRVQSNGKPIRAFFAFDPERTGIILCGGDKTGDKRFYKKMIPIAD
AEFDEHLEELKNEK

Nucleotide

• Length: 285 bp

>T65775 NZ_CP016987:2344646-2344930 [Vibrio cholerae]
ATTAATGTCCGCGCATGCATCCAGGTTCTTCTTGAAAGAGGGCCTCAGCTGAGCAGACCATACGCTGACACTGTGGAAGA
TTCAAAGCACAGCAACATGAAAGAGTTGCGAGTGCAAAGCAATGGGAAACCTATACGAGCTTTCTTTGCTTTTGACCCAG
AAAGAACAGGAATTATCTTATGTGGCGGTGACAAAACTGGCGATAAGAGATTCTACAAAAAGATGATTCCGATAGCTGAT
GCAGAATTTGATGAGCATTTAGAGGAACTTAAAAATGAAAAGTAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 9658.26 Da
• Isoelectric Point: 7.2036

>AT65775 WP_032475673.1 NZ_CP016987:2344956-2345225 [Vibrio cholerae]
MSPEALAAAKEKAADMLLDIRLAELREITHLSQVEIAKAMGISQPSVANLEKSGKDLRLSSLKKYIEATGGKLRIDVELP
DGKHFGFPV

Nucleotide

• Length: 270 bp

>AT65775 NZ_CP016987:2344956-2345225 [Vibrio cholerae]
ATGTCACCAGAAGCACTTGCTGCCGCTAAAGAAAAAGCAGCGGATATGCTTCTTGATATTCGACTTGCTGAGCTTCGAGA
GATCACCCATCTATCTCAAGTCGAGATCGCTAAAGCCATGGGCATTAGTCAGCCATCAGTTGCCAATTTAGAGAAATCTG
GCAAAGATTTACGTCTTAGCTCTCTAAAGAAATACATCGAAGCAACCGGTGGTAAGCTTCGCATTGATGTAGAACTGCCA
GACGGAAAGCATTTTGGCTTCCCAGTGTAG