65757

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) unclassified/-
Location 2989750..2990398 Replicon chromosome
Accession NZ_CP016972
Organism Mycobacterium tuberculosis H37Ra

Toxin (Protein)


Gene name novel[antitoxin] Uniprot ID P9WJ12
Locus tag BB988_RS14695 Protein ID WP_003899414.1
Coordinates 2989750..2990073 (-) Length 108 a.a.

Antitoxin (Protein)


Gene name novel[toxin] Uniprot ID P9WJ10
Locus tag BB988_RS14700 Protein ID WP_003899415.1
Coordinates 2990153..2990398 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BB988_RS14670 2985324..2986585 + 1262 WP_087902221.1 IS3-like element IS987 family transposase -
BB988_RS14680 2986959..2988398 - 1440 WP_009935349.1 phage major capsid protein -
BB988_RS14685 2988406..2988939 - 534 WP_003899412.1 HK97 family phage prohead protease -
BB988_RS14690 2989092..2989583 - 492 WP_003900541.1 phage terminase small subunit P27 family -
BB988_RS14695 2989750..2990073 - 324 WP_003899414.1 type II toxin-antitoxin system toxin Toxin
BB988_RS14700 2990153..2990398 - 246 WP_003899415.1 type II toxin-antitoxin system antitoxin Antitoxin
BB988_RS14705 2990395..2991822 - 1428 WP_003899416.1 DUF3631 domain-containing protein -
BB988_RS14710 2991824..2992216 - 393 WP_003899417.1 DUF2742 domain-containing protein -
BB988_RS14715 2992213..2992473 - 261 WP_003899418.1 helix-turn-helix domain-containing protein -
BB988_RS14720 2992490..2992852 - 363 WP_003900543.1 hypothetical protein -
BB988_RS14725 2992855..2993982 - 1128 WP_003899420.1 site-specific integrase -
BB988_RS14730 2994127..2994354 - 228 WP_003899421.1 hypothetical protein -
BB988_RS14735 2994351..2994740 - 390 WP_003899422.1 hypothetical protein -
BB988_RS14740 2994646..2994918 + 273 WP_003900544.1 hypothetical protein -
BB988_RS14745 2995017..2995250 + 234 WP_003413717.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 2983715..2993982 10267


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-127)

Antitoxin

Arc-like DNA binding domain

(1-39)


Sequences

Toxin        


Download         Length: 108 a.a.        Molecular weight: 12359.82 Da        Isoelectric Point: 8.6717

>T65757 WP_003899414.1 NZ_CP016972:c2990073-2989750 [Mycobacterium tuberculosis H37Ra]
MTHKRTKRQPAIAAGLNAPRRNRVGRQHGWPADVPSAEQRRAQRQRDLEAIRRAYAEMVATSHEIDDDTAELALLSMHLD
DEQRRLEAGMKLGWHPYHFPDEPDSKQ

Download         Length: 324 bp

>T65757 NZ_CP016972:c2990073-2989750 [Mycobacterium tuberculosis H37Ra]
TTGACCCACAAGCGCACTAAACGCCAGCCAGCCATCGCCGCAGGGCTCAACGCCCCGCGTCGGAATCGCGTTGGGCGGCA
ACATGGTTGGCCGGCCGACGTTCCGTCCGCCGAGCAGCGCCGCGCCCAACGGCAGCGCGACCTCGAGGCTATCCGCCGAG
CGTACGCCGAGATGGTGGCGACATCACACGAAATCGACGACGACACAGCCGAACTGGCGCTGTTGTCGATGCATCTCGAC
GATGAGCAGCGCCGGCTTGAGGCGGGGATGAAGCTCGGCTGGCATCCGTATCACTTCCCCGACGAACCCGACAGCAAACA
GTGA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 7697.71 Da        Isoelectric Point: 4.8565

>AT65757 WP_003899415.1 NZ_CP016972:c2990398-2990153 [Mycobacterium tuberculosis H37Ra]
MSGHALAARTLLAAADELVGGPPVEASAAALAGDAAGAWRTAAVELARALVRAVAESHGVAAVLFAATAAAAAAVDRGDP
P

Download         Length: 246 bp

>AT65757 NZ_CP016972:c2990398-2990153 [Mycobacterium tuberculosis H37Ra]
ATGAGCGGCCACGCGTTGGCTGCTCGGACGTTGCTGGCCGCCGCGGACGAGCTTGTCGGCGGCCCGCCAGTCGAGGCTTC
GGCCGCCGCGCTGGCCGGCGACGCCGCGGGCGCATGGCGGACCGCGGCCGTCGAGCTTGCGCGAGCGTTGGTCCGCGCTG
TGGCGGAGTCGCACGGCGTCGCGGCCGTTTTGTTCGCCGCGACGGCCGCCGCGGCGGCGGCCGTCGACCGGGGTGATCCG
CCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A045IHC4

Antitoxin

Source ID Structure
AlphaFold DB A0A806JR81

References