Detailed information of TA system TA065674

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 1095756..1096351
• Replicon: chromosome
• Accession: NZ_CP016955
• Organism: Pseudomonas aeruginosa strain RIVM-EMC2982

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A241XLJ5
• Locus tag: BFV99_RS05030
• Protein ID: WP_003117425.1
• Coordinates: 1095756..1096034 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: BFV99_RS05035
• Protein ID: WP_003099268.1
• Coordinates: 1096046..1096351 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BFV99_RS05015	1091186..1092424	+	1239	WP_014603324.1	C69 family dipeptidase	-
BFV99_RS05020	1092486..1093133	+	648	WP_003095021.1	carbonate dehydratase	-
BFV99_RS05025	1093203..1095431	-	2229	WP_003113525.1	TonB-dependent receptor	-
BFV99_RS05030	1095756..1096034	+	279	WP_003117425.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BFV99_RS05035	1096046..1096351	+	306	WP_003099268.1	HigA family addiction module antidote protein	Antitoxin
BFV99_RS05040	1096688..1097080	+	393	WP_025297673.1	hypothetical protein	-
BFV99_RS35880	1097141..1098232	-	1092	WP_124135598.1	hypothetical protein	-
BFV99_RS05045	1098285..1098620	-	336	WP_025297674.1	hypothetical protein	-
BFV99_RS05055	1099067..1099339	+	273	WP_004352675.1	hypothetical protein	-
BFV99_RS05060	1099515..1099805	+	291	WP_033896069.1	DUF5447 family protein	-
BFV99_RS05070	1100321..1100755	+	435	WP_023087663.1	hypothetical protein	-
BFV99_RS05080	1100877..1101128	+	252	WP_003115979.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-97)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10701.28 Da
• Isoelectric Point: 8.5576

>T65674 WP_003117425.1 NZ_CP016955:1095756-1096034 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAARELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T65674 NZ_CP016955:1095756-1096034 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTAGGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11213.79 Da
• Isoelectric Point: 5.0747

>AT65674 WP_003099268.1 NZ_CP016955:1096046-1096351 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT65674 NZ_CP016955:1096046-1096351 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTGGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: A0A241XLJ5