Detailed information of TA system TA065655

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 1090187..1090835
• Replicon: chromosome
• Accession: NZ_CP016952
• Organism: Citrobacter freundii strain SL151

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: BFQ28_RS05125
• Protein ID: WP_046671055.1
• Coordinates: 1090187..1090549 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A0D7LEH2
• Locus tag: BFQ28_RS05130
• Protein ID: WP_003840660.1
• Coordinates: 1090536..1090835 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BFQ28_RS05110	1086646..1087974	+	1329	WP_003023927.1	MFS transporter	-
BFQ28_RS05115	1088117..1089508	+	1392	WP_003023929.1	hexose-6-phosphate:phosphate antiporter	-
BFQ28_RS05120	1089634..1090086	+	453	WP_003023933.1	DUF1198 family protein	-
BFQ28_RS05125	1090187..1090549	+	363	WP_046671055.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BFQ28_RS05130	1090536..1090835	+	300	WP_003840660.1	helix-turn-helix transcriptional regulator	Antitoxin
BFQ28_RS05135	1090954..1092144	+	1191	WP_032937137.1	purine ribonucleoside efflux pump NepI	-
BFQ28_RS05140	1092194..1093576	-	1383	WP_046671054.1	glycoside hydrolase family 1 protein	-
BFQ28_RS05145	1093671..1093964	-	294	WP_003840665.1	hypothetical protein	-
BFQ28_RS05150	1094095..1094511	+	417	WP_003023951.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-107)

Antitoxin

• Cro/C1-type helix-turn-helix domain (31-84)

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13814.69 Da
• Isoelectric Point: 4.9208

>T65655 WP_046671055.1 NZ_CP016952:1090187-1090549 [Citrobacter freundii]
MWEVETTDAFDKWFDVQTEALKEDMLAAMMILSEYGPQLGRPFADTVNASAFSNMKELRVQHQGSPIRAFFAFDPSRYGI
VLCAGDKTGLNEKKFYKEMIRLADAEYRNHLISKENYGYP

Nucleotide

• Length: 363 bp

>T65655 NZ_CP016952:1090187-1090549 [Citrobacter freundii]
ATGTGGGAAGTGGAAACAACGGATGCTTTCGATAAATGGTTTGACGTACAGACCGAAGCGCTCAAGGAAGATATGCTCGC
AGCAATGATGATTTTATCCGAGTATGGTCCTCAGTTAGGTAGGCCGTTTGCTGATACGGTAAATGCTTCTGCTTTTTCAA
ATATGAAAGAGTTGCGAGTACAGCATCAGGGTAGTCCGATTCGGGCGTTCTTCGCGTTTGATCCTTCTCGCTATGGCATC
GTGCTATGCGCTGGAGATAAAACAGGGTTAAACGAAAAGAAATTCTATAAAGAGATGATCAGGCTTGCGGATGCTGAGTA
CCGAAATCACCTAATAAGTAAGGAAAATTATGGCTACCCTTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11016.85 Da
• Isoelectric Point: 8.5047

>AT65655 WP_003840660.1 NZ_CP016952:1090536-1090835 [Citrobacter freundii]
MATLKELMAKQSPESQERITEKVEILRQAVALNMLREELNLSQAELASAMGVKQPTIAKMEQADNDPRLSTLKRYVTALG
GELSIDVKLPTGKRVAFYL

Nucleotide

• Length: 300 bp

>AT65655 NZ_CP016952:1090536-1090835 [Citrobacter freundii]
ATGGCTACCCTTAAAGAATTGATGGCGAAACAAAGCCCGGAAAGCCAGGAGCGTATTACCGAAAAAGTAGAAATACTACG
TCAGGCGGTTGCGTTAAATATGTTAAGGGAAGAACTGAATCTCTCTCAGGCTGAACTTGCCTCGGCAATGGGGGTTAAGC
AGCCAACAATCGCAAAAATGGAGCAGGCAGATAACGATCCTCGTCTGTCGACGCTCAAGCGTTATGTCACGGCACTGGGT
GGTGAACTTAGCATTGATGTTAAGCTACCTACAGGTAAAAGAGTCGCGTTCTATCTTTAA

Structures

Antitoxin

• AlphaFold DB: A0A0D7LEH2