Detailed information of TA system TA065652

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 586613..587283
• Replicon: chromosome
• Accession: NZ_CP016952
• Organism: Citrobacter freundii strain SL151

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A6N6JXY6
• Locus tag: BFQ28_RS02735
• Protein ID: WP_046671374.1
• Coordinates: 586981..587283 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: BFQ28_RS02730
• Protein ID: WP_046671375.1
• Coordinates: 586613..586954 (-)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BFQ28_RS02700	582154..582912	+	759	WP_003844769.1	phosphonate C-P lyase system protein PhnK	-
BFQ28_RS02705	583002..583682	+	681	WP_046671387.1	phosphonate C-P lyase system protein PhnL	-
BFQ28_RS02710	583679..584815	+	1137	WP_003031811.1	alpha-D-ribose 1-methylphosphonate 5-triphosphate diphosphatase	-
BFQ28_RS02715	584818..585372	+	555	WP_003844764.1	ribose 1,5-bisphosphokinase	-
BFQ28_RS02720	585359..585793	+	435	WP_003031809.1	aminoalkylphosphonate N-acetyltransferase	-
BFQ28_RS02725	585802..586560	+	759	WP_046671376.1	phosphonate metabolism protein PhnP	-
BFQ28_RS02730	586613..586954	-	342	WP_046671375.1	HigA family addiction module antidote protein	Antitoxin
BFQ28_RS02735	586981..587283	-	303	WP_046671374.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BFQ28_RS02740	587439..587768	-	330	WP_003031807.1	hypothetical protein	-
BFQ28_RS02745	587838..590102	-	2265	WP_044702173.1	hybrid sensor histidine kinase/response regulator	-
BFQ28_RS02750	590217..591740	+	1524	WP_003844755.1	sugar ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (9-98)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-70)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11819.43 Da
• Isoelectric Point: 9.9581

>T65652 WP_046671374.1 NZ_CP016952:c587283-586981 [Citrobacter freundii]
MSKSLNIRSFRDTWLEDFFERATSHRKIPADIHTALARKLDIINAAVSHRDLRSPPGNRYEELTGKLQEYSSIRVNKQYR
LIFKWVNGKAEDVYLDPHIY

Nucleotide

• Length: 303 bp

>T65652 NZ_CP016952:c587283-586981 [Citrobacter freundii]
ATGTCTAAATCATTAAATATCAGGAGCTTTCGGGATACATGGCTTGAAGATTTTTTTGAGCGGGCGACATCGCACAGAAA
AATACCAGCGGATATTCATACTGCATTAGCCAGAAAGCTGGATATCATTAATGCGGCTGTTTCGCATCGGGATTTGCGCT
CGCCACCCGGTAACAGATATGAAGAGTTAACCGGAAAATTACAGGAATATTCTTCAATCAGAGTAAACAAGCAGTACCGA
TTAATATTTAAATGGGTTAACGGTAAGGCCGAAGATGTGTACTTAGATCCACACATCTACTAG

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 13017.84 Da
• Isoelectric Point: 7.2040

>AT65652 WP_046671375.1 NZ_CP016952:c586954-586613 [Citrobacter freundii]
MKQATRKPTTVGDILQYEYLEPLGLKINDLAEILHVHRNTVSALVNNNRKLTTDMAFRLAKAFDTSVDFWRNLQAAVDLW
EVENDMRAQEELNRIVSVKDFIAQRNSESKKVA

Nucleotide

• Length: 342 bp

>AT65652 NZ_CP016952:c586954-586613 [Citrobacter freundii]
ATGAAACAGGCAACCAGAAAACCAACGACTGTGGGTGATATCCTGCAATATGAATACCTGGAGCCGCTCGGACTGAAAAT
TAACGATCTGGCTGAAATACTGCATGTTCATCGCAATACGGTGAGCGCACTGGTCAACAATAATCGCAAATTAACAACGG
ATATGGCGTTTCGTTTGGCCAAAGCGTTCGATACCTCCGTTGATTTCTGGCGTAACTTACAGGCGGCGGTTGACCTGTGG
GAAGTTGAAAATGACATGCGGGCGCAAGAAGAACTGAACCGCATTGTTTCGGTTAAAGATTTTATCGCTCAGCGAAATAG
CGAGTCTAAAAAAGTCGCCTGA

Structures

Toxin

• AlphaFold DB: A0A6N6JXY6