Detailed information of TA system TA065638

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 5005752..5006405
• Replicon: chromosome
• Accession: NZ_CP016948
• Organism: Serratia surfactantfaciens strain YD25

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: ATE40_RS23370
• Protein ID: WP_033645671.1
• Coordinates: 5005752..5006102 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A5C7DBM3
• Locus tag: ATE40_RS23375
• Protein ID: WP_019453270.1
• Coordinates: 5006106..5006405 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ATE40_RS23335	5000908..5001318	-	411	WP_063918079.1	GNAT family N-acetyltransferase	-
ATE40_RS23340	5001351..5002721	-	1371	WP_063918080.1	glutamine synthetase family protein	-
ATE40_RS23345	5002958..5003275	+	318	WP_063918081.1	hypothetical protein	-
ATE40_RS23350	5003266..5003661	-	396	WP_063918082.1	hypothetical protein	-
ATE40_RS23355	5003675..5004067	-	393	WP_063918083.1	hypothetical protein	-
ATE40_RS23360	5004095..5004484	-	390	WP_063918084.1	hypothetical protein	-
ATE40_RS23365	5004484..5005488	-	1005	WP_063918085.1	hypothetical protein	-
ATE40_RS23370	5005752..5006102	+	351	WP_033645671.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ATE40_RS23375	5006106..5006405	+	300	WP_019453270.1	XRE family transcriptional regulator	Antitoxin
ATE40_RS23380	5006395..5007312	-	918	WP_063918086.1	LysR family transcriptional regulator	-
ATE40_RS23385	5007481..5008179	+	699	WP_004933280.1	CoA transferase subunit A	-
ATE40_RS23390	5008191..5008844	+	654	WP_015376496.1	CoA transferase subunit B	-
ATE40_RS23395	5008859..5010043	+	1185	WP_019453267.1	acetyl-CoA C-acetyltransferase	-
ATE40_RS23400	5010061..5010984	+	924	WP_019453266.1	3-hydroxyacyl-CoA dehydrogenase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-83)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13546.81 Da
• Isoelectric Point: 8.2699

>T65638 WP_033645671.1 NZ_CP016948:5005752-5006102 [Serratia surfactantfaciens]
MWTVMTTEEFDRWLCEQDESTQEKVLAALVMLERAGPSLRRPFVDVLKGSLHPNMKELRIQHKGRPIRAFFAFDPARQAI
VLCAGDKTGNEKRFYKVMLPIADAQFTQYLMCYFKE

Nucleotide

• Length: 351 bp

>T65638 NZ_CP016948:5005752-5006102 [Serratia surfactantfaciens]
ATGTGGACGGTAATGACAACGGAAGAGTTTGATCGGTGGCTCTGTGAGCAGGATGAATCGACGCAGGAAAAGGTGTTGGC
CGCGCTGGTGATGCTCGAGCGTGCCGGGCCTTCTCTTCGGCGGCCTTTTGTCGACGTGCTGAAAGGCTCGTTGCATCCCA
ACATGAAAGAGCTGCGTATTCAGCACAAAGGGCGGCCGATTCGCGCTTTTTTCGCCTTCGATCCGGCGCGGCAGGCGATT
GTGCTGTGTGCGGGCGACAAAACGGGTAATGAGAAGCGGTTTTATAAAGTGATGCTGCCCATCGCGGATGCGCAGTTCAC
CCAATATTTGATGTGTTATTTCAAGGAGTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11013.71 Da
• Isoelectric Point: 7.3207

>AT65638 WP_019453270.1 NZ_CP016948:5006106-5006405 [Serratia surfactantfaciens]
MSTLKQLLASRTPESRRRIEEMAEEMLFEAHLHQLREALNISQQELAKTLGISQPSVAAIEGRGNELKISTLKRYVEAMG
GTLRLDVELPGGKHVGFTV

Nucleotide

• Length: 300 bp

>AT65638 NZ_CP016948:5006106-5006405 [Serratia surfactantfaciens]
ATGAGCACCTTAAAGCAACTGCTGGCCTCCCGCACCCCGGAGAGCCGCCGGCGCATCGAGGAAATGGCCGAAGAGATGCT
GTTTGAAGCGCACCTGCACCAGCTGCGGGAAGCGCTGAATATTTCCCAACAGGAACTGGCGAAAACGCTGGGCATTTCCC
AGCCTTCTGTCGCGGCCATCGAAGGGCGCGGCAATGAGCTGAAGATTTCGACCCTGAAACGCTATGTGGAAGCGATGGGC
GGCACGCTGCGCCTGGACGTTGAGCTGCCGGGCGGCAAGCACGTCGGGTTTACGGTTTAA

Structures

Antitoxin

• AlphaFold DB: A0A5C7DBM3