Detailed information of TA system TA065564

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 551196..551779
• Replicon: chromosome
• Accession: NZ_CP016940
• Organism: Yersinia enterocolitica strain YE5

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7U7FJS5
• Locus tag: BED32_RS02845
• Protein ID: WP_005162526.1
• Coordinates: 551501..551779 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: BED32_RS02840
• Protein ID: WP_049525376.1
• Coordinates: 551196..551501 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BED32_RS02825	547012..547752	+	741	WP_049525374.1	KDGP aldolase family protein	-
BED32_RS02830	547882..549123	+	1242	Protein_463	lactonase family protein	-
BED32_RS02835	549196..551124	+	1929	WP_072079298.1	BglG family transcription antiterminator	-
BED32_RS02840	551196..551501	-	306	WP_049525376.1	HigA family addiction module antidote protein	Antitoxin
BED32_RS02845	551501..551779	-	279	WP_005162526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BED32_RS02850	551859..554581	-	2723	Protein_467	magnesium-translocating P-type ATPase	-
BED32_RS02855	555083..556030	+	948	WP_049525377.1	trehalose operon repressor TreR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (3-81)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10694.10 Da
• Isoelectric Point: 7.4011

>T65564 WP_005162526.1 NZ_CP016940:c551779-551501 [Yersinia enterocolitica]
MIKSWKHKGLQRFFETGSAAGINPQHVKRIRDRLRFIDLAETIEDVNVAGYKLHPLQGEREGIWSVTVSGNWRITFEFTA
GDAFILNYEDYH

Nucleotide

• Length: 279 bp

>T65564 NZ_CP016940:c551779-551501 [Yersinia enterocolitica]
ATGATAAAAAGTTGGAAACACAAAGGACTGCAACGCTTCTTTGAAACAGGTTCTGCCGCGGGCATTAATCCCCAGCATGT
TAAGCGAATTCGAGACCGCTTGCGATTTATCGACCTGGCGGAAACGATTGAGGATGTCAATGTTGCCGGATACAAGCTCC
ATCCGTTACAAGGTGAAAGAGAAGGGATCTGGTCAGTAACAGTAAGCGGAAATTGGCGCATTACATTCGAGTTTACCGCT
GGAGATGCGTTTATTCTTAACTACGAGGACTACCACTAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 10950.77 Da
• Isoelectric Point: 6.7158

>AT65564 WP_049525376.1 NZ_CP016940:c551501-551196 [Yersinia enterocolitica]
MKMHNPPHPGEMIEGILEDLGLGIREFARALNVSPSTVQRLVSCKAAISPEMAVKLAAVLGSTPAMWLRLQAAYDLGKAE
ENVDVSNLTKLYKPDPISLHS

Nucleotide

• Length: 306 bp

>AT65564 NZ_CP016940:c551501-551196 [Yersinia enterocolitica]
ATGAAAATGCATAATCCCCCACATCCAGGGGAAATGATTGAAGGAATCCTCGAAGATTTGGGTCTAGGGATAAGGGAGTT
TGCCAGGGCATTAAACGTGTCACCTTCAACGGTACAACGGCTGGTTTCCTGTAAGGCGGCAATTTCACCGGAAATGGCGG
TAAAACTGGCCGCGGTTCTGGGAAGTACCCCAGCAATGTGGCTCCGCCTCCAGGCCGCTTATGACCTGGGAAAAGCAGAA
GAGAATGTTGATGTATCCAATCTGACTAAACTGTACAAACCGGATCCAATATCGCTGCATAGTTGA

Structures

Toxin

• AlphaFold DB: A0A7U7FJS5