Detailed information of TA system TA065547

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1245973..1246556
• Replicon: chromosome
• Accession: NZ_CP016937
• Organism: Yersinia enterocolitica strain YE6

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7U7FJS5
• Locus tag: BED33_RS05535
• Protein ID: WP_005162526.1
• Coordinates: 1246278..1246556 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: BED33_RS05530
• Protein ID: WP_049525376.1
• Coordinates: 1245973..1246278 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BED33_RS05515	1241789..1242529	+	741	WP_049525374.1	KDGP aldolase family protein	-
BED33_RS05520	1242659..1243900	+	1242	Protein_1063	lactonase family protein	-
BED33_RS05525	1243973..1245901	+	1929	WP_072079298.1	BglG family transcription antiterminator	-
BED33_RS05530	1245973..1246278	-	306	WP_049525376.1	HigA family addiction module antidote protein	Antitoxin
BED33_RS05535	1246278..1246556	-	279	WP_005162526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BED33_RS05540	1246636..1249358	-	2723	Protein_1067	magnesium-translocating P-type ATPase	-
BED33_RS05545	1249860..1250807	+	948	WP_049525377.1	trehalose operon repressor TreR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (3-81)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10694.10 Da
• Isoelectric Point: 7.4011

>T65547 WP_005162526.1 NZ_CP016937:c1246556-1246278 [Yersinia enterocolitica]
MIKSWKHKGLQRFFETGSAAGINPQHVKRIRDRLRFIDLAETIEDVNVAGYKLHPLQGEREGIWSVTVSGNWRITFEFTA
GDAFILNYEDYH

Nucleotide

• Length: 279 bp

>T65547 NZ_CP016937:c1246556-1246278 [Yersinia enterocolitica]
ATGATAAAAAGTTGGAAACACAAAGGACTGCAACGCTTCTTTGAAACAGGTTCTGCCGCGGGCATTAATCCCCAGCATGT
TAAGCGAATTCGAGACCGCTTGCGATTTATCGACCTGGCGGAAACGATTGAGGATGTCAATGTTGCCGGATACAAGCTCC
ATCCGTTACAAGGTGAAAGAGAAGGGATCTGGTCAGTAACAGTAAGCGGAAATTGGCGCATTACATTCGAGTTTACCGCT
GGAGATGCGTTTATTCTTAACTACGAGGACTACCACTAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 10950.77 Da
• Isoelectric Point: 6.7158

>AT65547 WP_049525376.1 NZ_CP016937:c1246278-1245973 [Yersinia enterocolitica]
MKMHNPPHPGEMIEGILEDLGLGIREFARALNVSPSTVQRLVSCKAAISPEMAVKLAAVLGSTPAMWLRLQAAYDLGKAE
ENVDVSNLTKLYKPDPISLHS

Nucleotide

• Length: 306 bp

>AT65547 NZ_CP016937:c1246278-1245973 [Yersinia enterocolitica]
ATGAAAATGCATAATCCCCCACATCCAGGGGAAATGATTGAAGGAATCCTCGAAGATTTGGGTCTAGGGATAAGGGAGTT
TGCCAGGGCATTAAACGTGTCACCTTCAACGGTACAACGGCTGGTTTCCTGTAAGGCGGCAATTTCACCGGAAATGGCGG
TAAAACTGGCCGCGGTTCTGGGAAGTACCCCAGCAATGTGGCTCCGCCTCCAGGCCGCTTATGACCTGGGAAAAGCAGAA
GAGAATGTTGATGTATCCAATCTGACTAAACTGTACAAACCGGATCCAATATCGCTGCATAGTTGA

Structures

Toxin

• AlphaFold DB: A0A7U7FJS5