Detailed information of TA system TA065543

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 227499..228001
• Replicon: chromosome
• Accession: NZ_CP016937
• Organism: Yersinia enterocolitica strain YE6

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: -
• Locus tag: BED33_RS01020
• Protein ID: WP_049528435.1
• Coordinates: 227499..227753 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: A0A7U7IZ70
• Locus tag: BED33_RS01025
• Protein ID: WP_049528437.1
• Coordinates: 227750..228001 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BED33_RS01010	222589..224721	-	2133	WP_069009560.1	TonB-dependent siderophore receptor	-
BED33_RS01015	225140..227475	+	2336	Protein_197	mechanosensitive ion channel family protein	-
BED33_RS01020	227499..227753	-	255	WP_049528435.1	Txe/YoeB family addiction module toxin	Toxin
BED33_RS01025	227750..228001	-	252	WP_049528437.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
BED33_RS01030	228132..230917	-	2786	Protein_200	insulinase family protein	-
BED33_RS01035	230902..232647	-	1746	WP_162489085.1	ABC transporter ATP-binding protein/permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (2-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10098.54 Da
• Isoelectric Point: 8.4816

>T65543 WP_049528435.1 NZ_CP016937:c227753-227499 [Yersinia enterocolitica]
VRIIFSSCSWEDYLYWQQTDKEILKRINELVKNIQRTPFEGKGKPEPLKHNLAGFWSRRITEEHRLVYEVSGDNLLIAAC
RYHY

Nucleotide

• Length: 255 bp

>T65543 NZ_CP016937:c227753-227499 [Yersinia enterocolitica]
GTGAGAATTATATTTTCCAGTTGTTCTTGGGAGGATTATCTTTATTGGCAACAAACGGATAAGGAAATCCTCAAACGCAT
TAATGAGTTAGTAAAAAATATTCAAAGAACGCCATTTGAGGGAAAGGGTAAACCGGAACCCCTTAAACATAATCTGGCAG
GGTTCTGGTCACGGAGGATTACAGAAGAGCACAGACTTGTTTATGAGGTTTCCGGTGATAATTTATTAATTGCTGCTTGT
CGTTACCATTATTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9463.66 Da
• Isoelectric Point: 4.4878

>AT65543 WP_049528437.1 NZ_CP016937:c228001-227750 [Yersinia enterocolitica]
MRTISYSEARQNLSTTMVQTVEDRAPILITRQNGTSCVLMSLEEYESLEETAYLLRSPSNAKHLMDSIEELRAGKGIQRE
LEE

Nucleotide

• Length: 252 bp

>AT65543 NZ_CP016937:c228001-227750 [Yersinia enterocolitica]
ATGAGAACAATTAGTTATAGTGAGGCGCGCCAGAATTTGTCGACAACGATGGTGCAAACGGTTGAGGATCGAGCCCCCAT
CCTCATCACTCGTCAAAATGGGACTTCTTGTGTTCTTATGTCACTTGAAGAATATGAATCATTGGAAGAAACTGCTTATT
TACTGCGCTCACCATCAAACGCGAAGCATTTGATGGACTCAATTGAAGAGTTGAGAGCAGGAAAAGGAATTCAAAGGGAA
CTTGAAGAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A7U7IZ70