Detailed information of TA system TA065492

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 550956..551650
• Replicon: chromosome
• Accession: NZ_CP016928
• Organism: Dickeya solani RNS 08.23.3.1.A

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: -
• Locus tag: D083_RS02345
• Protein ID: WP_026594479.1
• Coordinates: 550956..551354 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: A0A2K8VUD1
• Locus tag: D083_RS02350
• Protein ID: WP_022633740.1
• Coordinates: 551357..551650 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D083_RS02315	546136..547170	-	1035	WP_022633732.1	harpin HrpZ family protein	-
D083_RS02320	547331..547741	-	411	WP_022633733.1	hypothetical protein	-
D083_RS02325	547738..547926	-	189	WP_022633734.1	hypothetical protein	-
D083_RS02330	547961..550030	-	2070	WP_023637836.1	type III secretion system outer membrane ring subunit SctC	-
D083_RS02335	550023..550457	-	435	WP_022633737.1	hypothetical protein	-
D083_RS02340	550444..550671	-	228	WP_022633738.1	type III secretion protein HrpF	-
D083_RS02345	550956..551354	-	399	WP_026594479.1	type II toxin-antitoxin system YafO family toxin	Toxin
D083_RS02350	551357..551650	-	294	WP_022633740.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
D083_RS02355	551868..552917	-	1050	WP_022633741.1	hypothetical protein	-
D083_RS02360	553457..554617	+	1161	WP_022633742.1	alpha/beta fold hydrolase	-
D083_RS02365	554628..555941	-	1314	WP_022633743.1	lytic murein transglycosylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	550444..560372	9928

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15230.56 Da
• Isoelectric Point: 8.4931

>T65492 WP_026594479.1 NZ_CP016928:c551354-550956 [Dickeya solani RNS 08.23.3.1.A]
IRIFKSTLIRQQLSQQELDDLVADFHAYKQNGVLPDTFGRDAPYDDDRTYPLVKAEQVAHIHLADADAPFPRFLRQFKRT
SDRAHLVYCQGAMDPEACLLIIILKPEAHKMARNNNNMHKIGMMAAAFRMKY

Nucleotide

• Length: 399 bp

>T65492 NZ_CP016928:c551354-550956 [Dickeya solani RNS 08.23.3.1.A]
ATACGGATTTTCAAGTCAACGCTGATCCGTCAACAATTGAGCCAGCAAGAGCTTGATGATTTGGTCGCGGATTTTCACGC
CTATAAGCAGAATGGGGTACTACCGGACACATTTGGGCGTGACGCGCCTTATGATGATGACCGAACCTATCCATTAGTAA
AAGCAGAGCAGGTGGCTCACATCCACCTTGCCGATGCGGATGCGCCTTTTCCCAGGTTCTTGCGCCAGTTCAAGAGAACC
AGCGATCGGGCCCATTTGGTCTACTGCCAGGGAGCGATGGATCCCGAGGCCTGCCTGTTAATTATTATTTTAAAACCCGA
AGCCCATAAAATGGCACGCAACAATAACAACATGCACAAGATCGGGATGATGGCCGCTGCGTTCAGGATGAAATATTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10934.48 Da
• Isoelectric Point: 5.2082

>AT65492 WP_022633740.1 NZ_CP016928:c551650-551357 [Dickeya solani RNS 08.23.3.1.A]
MERILAEKSINITELRKNPAKYFIDEPVAVLSNNRPAGYMVSAKVFEELIDLLEAKQETVHTVARFRPTAERLRALADSG
QKLLNDASGKDLTEFTE

Nucleotide

• Length: 294 bp

>AT65492 NZ_CP016928:c551650-551357 [Dickeya solani RNS 08.23.3.1.A]
ATGGAGCGTATTCTTGCTGAAAAATCTATAAATATTACAGAGTTAAGGAAAAATCCGGCTAAGTATTTTATTGATGAGCC
TGTTGCCGTGCTTTCTAACAACCGGCCCGCCGGTTACATGGTGAGTGCCAAAGTCTTTGAAGAACTGATCGATCTGCTAG
AGGCGAAGCAGGAAACGGTTCATACCGTAGCGCGCTTTAGGCCAACGGCCGAACGTTTACGAGCCCTTGCAGATAGCGGT
CAGAAACTGCTGAATGATGCCTCTGGCAAGGATCTGACTGAGTTCACGGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A2K8VUD1