Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 1296149..1296746 Replicon chromosome
Accession NZ_CP016926
Organism Klebsiella pneumoniae isolate 23

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag A8C23_RS07250 Protein ID WP_004142563.1
Coordinates 1296429..1296746 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag A8C23_RS07245 Protein ID WP_004142561.1
Coordinates 1296149..1296436 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A8C23_RS07215 1292229..1292477 + 249 WP_002893037.1 DUF1158 domain-containing protein -
A8C23_RS07220 1292495..1292836 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
A8C23_RS07225 1292867..1293982 - 1116 WP_020316605.1 MBL fold metallo-hydrolase -
A8C23_RS07230 1294162..1294743 + 582 WP_004176968.1 TetR/AcrR family transcriptional regulator -
A8C23_RS07235 1294743..1295111 + 369 WP_004142557.1 MmcQ/YjbR family DNA-binding protein -
A8C23_RS07240 1295231..1295884 + 654 WP_004178896.1 oxygen-insensitive NAD(P)H nitroreductase -
A8C23_RS07245 1296149..1296436 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
A8C23_RS07250 1296429..1296746 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
A8C23_RS07255 1296931..1297974 - 1044 WP_004178895.1 DUF2157 domain-containing protein -
A8C23_RS07260 1298641..1299507 - 867 WP_004178894.1 helix-turn-helix transcriptional regulator -
A8C23_RS07265 1299616..1301043 + 1428 WP_004176980.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T65468 WP_004142563.1 NZ_CP016926:c1296746-1296429 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T65468 NZ_CP016926:c1296746-1296429 [Klebsiella pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT65468 WP_004142561.1 NZ_CP016926:c1296436-1296149 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT65468 NZ_CP016926:c1296436-1296149 [Klebsiella pneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCAACGGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References