Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 3297420..3298017 Replicon chromosome
Accession NZ_CP016923
Organism Klebsiella pneumoniae isolate 11

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag A8C11_RS18330 Protein ID WP_004142563.1
Coordinates 3297420..3297737 (+) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag A8C11_RS18335 Protein ID WP_004142561.1
Coordinates 3297730..3298017 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A8C11_RS18315 3293123..3294550 - 1428 WP_043906839.1 MFS transporter -
A8C11_RS18320 3294659..3295525 + 867 WP_004178894.1 helix-turn-helix transcriptional regulator -
A8C11_RS18325 3296192..3297235 + 1044 WP_004178895.1 DUF2157 domain-containing protein -
A8C11_RS18330 3297420..3297737 + 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
A8C11_RS18335 3297730..3298017 + 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
A8C11_RS18340 3298285..3298935 - 651 Protein_3040 oxygen-insensitive NAD(P)H nitroreductase -
A8C11_RS18345 3299055..3299423 - 369 WP_004142557.1 MmcQ/YjbR family DNA-binding protein -
A8C11_RS18350 3299423..3300004 - 582 WP_043906838.1 TetR/AcrR family transcriptional regulator -
A8C11_RS18355 3300184..3301299 + 1116 WP_020316605.1 MBL fold metallo-hydrolase -
A8C11_RS18360 3301330..3301671 + 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
A8C11_RS18365 3301689..3301937 - 249 WP_002893037.1 DUF1158 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T65456 WP_004142563.1 NZ_CP016923:3297420-3297737 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T65456 NZ_CP016923:3297420-3297737 [Klebsiella pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT65456 WP_004142561.1 NZ_CP016923:3297730-3298017 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT65456 NZ_CP016923:3297730-3298017 [Klebsiella pneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCAACGGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References