Detailed information of TA system TA065442

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/MqsA(antitoxin)
• Location: 703157..703809
• Replicon: plasmid unnamed
• Accession: NZ_CP016915
• Organism: Ralstonia solanacearum strain CQPS-1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A0S4U5H4
• Locus tag: BC350_RS20950
• Protein ID: WP_028861315.1
• Coordinates: 703157..703501 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: BC350_RS20955
• Protein ID: WP_020425351.1
• Coordinates: 703498..703809 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BC350_RS20935	698853..700016	-	1164	WP_016726526.1	2-methylcitrate synthase	-
BC350_RS20940	700068..700964	-	897	WP_071092368.1	methylisocitrate lyase	-
BC350_RS20945	701157..703106	+	1950	WP_023470006.1	propionate catabolism operon regulatory protein PrpR	-
BC350_RS20950	703157..703501	+	345	WP_028861315.1	hypothetical protein	Toxin
BC350_RS20955	703498..703809	+	312	WP_020425351.1	transcriptional regulator	Antitoxin
BC350_RS20960	703949..704758	-	810	WP_071623829.1	DUF2242 domain-containing protein	-
BC350_RS20965	705402..706061	-	660	WP_149079161.1	hypothetical protein	-
BC350_RS20970	706390..708237	+	1848	WP_071623825.1	AAA family ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	cheY / cheB / cheW / motA / cyaB / flgC / flgG / fliM / fliG / fliI / adeG / tsr / acrB	1..2061090	2061090

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (41-91)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13276.03 Da
• Isoelectric Point: 9.3395

>T65442 WP_028861315.1 NZ_CP016915:703157-703501 [Ralstonia solanacearum]
MDATFIELPPFQRLREHYLSDDEYRALQQELLARPDAGDVIKGTGGLRKLRFSDKRRGKGKRGGLRVIYYHWDGGGQFWM
FVVYDKGEADDLSSDERKVLARLLEQEVKARSER

Nucleotide

• Length: 345 bp

>T65442 NZ_CP016915:703157-703501 [Ralstonia solanacearum]
ATGGATGCTACCTTTATCGAGTTGCCGCCTTTTCAGAGGCTGCGGGAGCACTATCTCTCGGACGACGAGTACCGAGCGCT
CCAGCAGGAACTGCTGGCCCGGCCGGACGCCGGCGACGTCATCAAGGGGACAGGTGGCCTACGCAAGCTGCGATTTTCGG
ACAAGCGGCGCGGCAAAGGTAAGCGCGGCGGTCTGCGCGTGATTTACTACCACTGGGATGGAGGCGGCCAATTCTGGATG
TTTGTCGTCTACGACAAGGGCGAGGCGGACGACCTTTCCAGCGACGAGCGCAAAGTGCTGGCACGGCTGCTGGAGCAGGA
AGTCAAGGCAAGGAGTGAGCGATGA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11619.65 Da
• Isoelectric Point: 10.7132

>AT65442 WP_020425351.1 NZ_CP016915:703498-703809 [Ralstonia solanacearum]
MKRNLFAELKEGMDALAAEREGKMTLRKVKVRVLQPLDVTAEEIKAVRAAAHASQAVMARRLRVNVRTYQNWEQGTAKPN
TQAAVLIKLVEKHPETLQMLEAL

Nucleotide

• Length: 312 bp

>AT65442 NZ_CP016915:703498-703809 [Ralstonia solanacearum]
ATGAAACGGAACCTGTTTGCTGAGTTGAAGGAGGGGATGGATGCGCTTGCCGCAGAGCGCGAAGGAAAGATGACCCTTCG
CAAGGTGAAGGTCCGCGTGCTCCAGCCGCTTGACGTGACAGCCGAAGAAATCAAGGCCGTTCGCGCAGCGGCACATGCTT
CACAGGCTGTCATGGCACGTCGACTGCGCGTCAATGTGCGCACCTACCAGAACTGGGAACAAGGAACCGCCAAACCGAAC
ACCCAGGCGGCTGTGTTGATCAAGCTCGTCGAGAAGCATCCGGAAACGTTGCAGATGCTGGAAGCGCTTTAA

Structures

Toxin

• AlphaFold DB: A0A0S4U5H4